| pubmed-article:16141335 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16141335 | lifeskim:mentions | umls-concept:C0011923 | lld:lifeskim |
| pubmed-article:16141335 | lifeskim:mentions | umls-concept:C0026018 | lld:lifeskim |
| pubmed-article:16141335 | lifeskim:mentions | umls-concept:C0016315 | lld:lifeskim |
| pubmed-article:16141335 | lifeskim:mentions | umls-concept:C1549650 | lld:lifeskim |
| pubmed-article:16141335 | lifeskim:mentions | umls-concept:C2699488 | lld:lifeskim |
| pubmed-article:16141335 | lifeskim:mentions | umls-concept:C1549481 | lld:lifeskim |
| pubmed-article:16141335 | pubmed:issue | 37 | lld:pubmed |
| pubmed-article:16141335 | pubmed:dateCreated | 2005-9-14 | lld:pubmed |
| pubmed-article:16141335 | pubmed:abstractText | Contrary to the well known diffraction limit, the fluorescence microscope is in principle capable of unlimited resolution. The necessary elements are spatially structured illumination light and a nonlinear dependence of the fluorescence emission rate on the illumination intensity. As an example of this concept, this article experimentally demonstrates saturated structured-illumination microscopy, a recently proposed method in which the nonlinearity arises from saturation of the excited state. This method can be used in a simple, wide-field (nonscanning) microscope, uses only a single, inexpensive laser, and requires no unusual photophysical properties of the fluorophore. The practical resolving power is determined by the signal-to-noise ratio, which in turn is limited by photobleaching. Experimental results show that a 2D point resolution of <50 nm is possible on sufficiently bright and photostable samples. | lld:pubmed |
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| pubmed-article:16141335 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16141335 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16141335 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16141335 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16141335 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:16141335 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:16141335 | pubmed:author | pubmed-author:GustafssonMat... | lld:pubmed |
| pubmed-article:16141335 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16141335 | pubmed:day | 13 | lld:pubmed |
| pubmed-article:16141335 | pubmed:volume | 102 | lld:pubmed |
| pubmed-article:16141335 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16141335 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16141335 | pubmed:pagination | 13081-6 | lld:pubmed |
| pubmed-article:16141335 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:16141335 | pubmed:meshHeading | pubmed-meshheading:16141335... | lld:pubmed |
| pubmed-article:16141335 | pubmed:meshHeading | pubmed-meshheading:16141335... | lld:pubmed |
| pubmed-article:16141335 | pubmed:meshHeading | pubmed-meshheading:16141335... | lld:pubmed |
| pubmed-article:16141335 | pubmed:meshHeading | pubmed-meshheading:16141335... | lld:pubmed |
| pubmed-article:16141335 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:16141335 | pubmed:articleTitle | Nonlinear structured-illumination microscopy: wide-field fluorescence imaging with theoretically unlimited resolution. | lld:pubmed |
| pubmed-article:16141335 | pubmed:affiliation | Department of Physiology and Program in Bioengineering, University of California, San Francisco, CA 94143-2532, USA. mats@msg.ucsf.edu | lld:pubmed |
| pubmed-article:16141335 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16141335 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:16141335 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:16141335 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:16141335 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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