| pubmed-article:16116089 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16116089 | lifeskim:mentions | umls-concept:C0675096 | lld:lifeskim |
| pubmed-article:16116089 | lifeskim:mentions | umls-concept:C0741847 | lld:lifeskim |
| pubmed-article:16116089 | lifeskim:mentions | umls-concept:C0441712 | lld:lifeskim |
| pubmed-article:16116089 | lifeskim:mentions | umls-concept:C0040092 | lld:lifeskim |
| pubmed-article:16116089 | pubmed:issue | 35 | lld:pubmed |
| pubmed-article:16116089 | pubmed:dateCreated | 2005-8-31 | lld:pubmed |
| pubmed-article:16116089 | pubmed:abstractText | UV-light-induced cyclobutane pyrimidine dimers (CPDs) present a severe block to synthesis by replicative DNA polymerases (Pols), whereas Poleta promotes proficient and error-free replication through CPDs. Although the archael Dpo4, which, like Poleta, belongs to the Y family of DNA Pols, can also replicate through a CPD, it is much less efficient than Poleta. The x-ray crystal structure of Dpo4 complexed with either the 3'-thymine (T) or the 5' T of a cis-syn TT dimer has indicated that, whereas the 3' T of the dimer forms a Watson-Crick base pair with the incoming dideoxy ATP, the 5' T forms a Hoogsteen base pair with the dideoxy ATP in syn conformation. Based upon these observations, a similar mechanism involving Hoogsteen base pairing of the 5' T of the dimer with the incoming A has been proposed for Poleta. Here we examine the mechanisms of CPD bypass by Dpo4 and Poleta using nucleotide analogs that specifically disrupt the Hoogsteen or Watson-Crick base pairing. Our results show that both Dpo4 and Poleta incorporate dATP opposite the 5' T of the CPD via Watson-Crick base pairing and not by Hoogsteen base pairing. Furthermore, opposite the 3' T of the dimer, the two Pols differ strikingly in the mechanisms of dATP incorporation, with Dpo4 incorporating opposite an abasic-like intermediate and Poleta using the normal Watson-Crick base pairing. These observations have important implications for the mechanisms used for the inefficient vs. efficient bypass of CPDs by DNA Pols. | lld:pubmed |
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| pubmed-article:16116089 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16116089 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16116089 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:16116089 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16116089 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:16116089 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:16116089 | pubmed:author | pubmed-author:JohnsonRobert... | lld:pubmed |
| pubmed-article:16116089 | pubmed:author | pubmed-author:PrakashLouise... | lld:pubmed |
| pubmed-article:16116089 | pubmed:author | pubmed-author:PrakashSatyaS | lld:pubmed |
| pubmed-article:16116089 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16116089 | pubmed:day | 30 | lld:pubmed |
| pubmed-article:16116089 | pubmed:volume | 102 | lld:pubmed |
| pubmed-article:16116089 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16116089 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16116089 | pubmed:pagination | 12359-64 | lld:pubmed |
| pubmed-article:16116089 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:16116089 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:16116089 | pubmed:articleTitle | Distinct mechanisms of cis-syn thymine dimer bypass by Dpo4 and DNA polymerase eta. | lld:pubmed |
| pubmed-article:16116089 | pubmed:affiliation | Sealy Center for Molecular Science, University of Texas Medical Branch, 6.104 Blocker Medical Research Building, Galveston, TX 77555-1061, USA. | lld:pubmed |
| pubmed-article:16116089 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16116089 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:16116089 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:16116089 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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