| pubmed-article:16041126 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16041126 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:16041126 | lifeskim:mentions | umls-concept:C0021528 | lld:lifeskim |
| pubmed-article:16041126 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:16041126 | lifeskim:mentions | umls-concept:C0332453 | lld:lifeskim |
| pubmed-article:16041126 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:16041126 | pubmed:dateCreated | 2005-7-25 | lld:pubmed |
| pubmed-article:16041126 | pubmed:abstractText | Using an inosine-producing mutant of Escherichia coli, the contributions of the central carbon metabolism for overproducing inosine were investigated. Sodium gluconate instead of glucose was tested as a carbon source to increase the supply of ribose-5-phosphate through the oxidative pentose phosphate pathway. The edd (6-phosphogluconate dehydrase gene)-disrupted mutant accumulated 2.5 g/l of inosine from 48 g/l of sodium gluconate, compared with 1.4 g/l of inosine in the edd wild strain. The rpe (ribulose phosphate 3-epimerase gene)-disrupted mutant resulted in low cell growth and low inosine production on glucose and on gluconate. The disruption of pgi (glucose-6-phosphate isomerase gene) was effective for increasing the accumulation of inosine from glucose but resulted in low cell growth. The pgi-disrupted mutant accumulated 3.7 g/l of inosine from 40 g/l of glucose when 8 g/l of yeast extract was added to the medium. Furthermore, to improve effective utilization of adenine, the yicP (adenine deaminase gene)-disrupted mutant was evaluated. It showed higher inosine accumulation, of 3.7 g/l, than that of 2.8 g/l in the yicP wild strain when 4 g/l of yeast extract was added to the medium. | lld:pubmed |
| pubmed-article:16041126 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16041126 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16041126 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16041126 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16041126 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16041126 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16041126 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16041126 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16041126 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16041126 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16041126 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16041126 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16041126 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16041126 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16041126 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:16041126 | pubmed:issn | 0916-8451 | lld:pubmed |
| pubmed-article:16041126 | pubmed:author | pubmed-author:TakenakaYasuh... | lld:pubmed |
| pubmed-article:16041126 | pubmed:author | pubmed-author:MatsuiHiroshi... | lld:pubmed |
| pubmed-article:16041126 | pubmed:author | pubmed-author:KawasakiHisas... | lld:pubmed |
| pubmed-article:16041126 | pubmed:author | pubmed-author:ShimaokaMegum... | lld:pubmed |
| pubmed-article:16041126 | pubmed:author | pubmed-author:KurahashiOsam... | lld:pubmed |
| pubmed-article:16041126 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16041126 | pubmed:volume | 69 | lld:pubmed |
| pubmed-article:16041126 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16041126 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16041126 | pubmed:pagination | 1248-55 | lld:pubmed |
| pubmed-article:16041126 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:16041126 | pubmed:meshHeading | pubmed-meshheading:16041126... | lld:pubmed |
| pubmed-article:16041126 | pubmed:meshHeading | pubmed-meshheading:16041126... | lld:pubmed |
| pubmed-article:16041126 | pubmed:meshHeading | pubmed-meshheading:16041126... | lld:pubmed |
| pubmed-article:16041126 | pubmed:meshHeading | pubmed-meshheading:16041126... | lld:pubmed |
| pubmed-article:16041126 | pubmed:meshHeading | pubmed-meshheading:16041126... | lld:pubmed |
| pubmed-article:16041126 | pubmed:meshHeading | pubmed-meshheading:16041126... | lld:pubmed |
| pubmed-article:16041126 | pubmed:meshHeading | pubmed-meshheading:16041126... | lld:pubmed |
| pubmed-article:16041126 | pubmed:meshHeading | pubmed-meshheading:16041126... | lld:pubmed |
| pubmed-article:16041126 | pubmed:meshHeading | pubmed-meshheading:16041126... | lld:pubmed |
| pubmed-article:16041126 | pubmed:meshHeading | pubmed-meshheading:16041126... | lld:pubmed |
| pubmed-article:16041126 | pubmed:meshHeading | pubmed-meshheading:16041126... | lld:pubmed |
| pubmed-article:16041126 | pubmed:meshHeading | pubmed-meshheading:16041126... | lld:pubmed |
| pubmed-article:16041126 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:16041126 | pubmed:articleTitle | Effects of edd and pgi disruptions on inosine accumulation in Escherichia coli. | lld:pubmed |
| pubmed-article:16041126 | pubmed:affiliation | Fermentation and Biotechnology Laboratories, Ajinomoto Co. Inc., 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki-shi, Kanagawa 210-8681, Japan. | lld:pubmed |
| pubmed-article:16041126 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16041126 | pubmed:publicationType | Comparative Study | lld:pubmed |
| entrez-gene:948210 | entrezgene:pubmed | pubmed-article:16041126 | lld:entrezgene |
| entrez-gene:947896 | entrezgene:pubmed | pubmed-article:16041126 | lld:entrezgene |
| entrez-gene:946362 | entrezgene:pubmed | pubmed-article:16041126 | lld:entrezgene |
| entrez-gene:948535 | entrezgene:pubmed | pubmed-article:16041126 | lld:entrezgene |
