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pubmed-article:16038613pubmed:dateCreated2005-7-25lld:pubmed
pubmed-article:16038613pubmed:abstractTextTwo extracellular oxidases (laccases) were isolated from the extracellular fluid of the fungus Panus (Lentinus) tigrinus cultivated in low-nitrogen medium supplemented with birch sawdust. The enzymes were purified by successive chromatography on columns with TEAE-cellulose and DEAE-Toyopearl 650M. Both oxidases catalyze oxidation of pyrocatechol and ABTS. Moreover, oxidase 1 also catalyzes oxidation of guaiacol, o-phenylenediamine, and syringaldazine. The enzymes have identical pH (7.0) and temperature (60-65 degrees C) optimums. Absorption spectra of the oxidases differ from the spectra of typical "blue" laccases and are similar to the spectrum of yellow oxidase.lld:pubmed
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pubmed-article:16038613pubmed:issn0006-2979lld:pubmed
pubmed-article:16038613pubmed:authorpubmed-author:SamuilovV DVDlld:pubmed
pubmed-article:16038613pubmed:authorpubmed-author:RevinV VVVlld:pubmed
pubmed-article:16038613pubmed:authorpubmed-author:CadimalievD...lld:pubmed
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pubmed-article:16038613pubmed:pagination703-7lld:pubmed
pubmed-article:16038613pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:16038613pubmed:year2005lld:pubmed
pubmed-article:16038613pubmed:articleTitleExtracellular oxidases of the lignin-degrading fungus Panus tigrinus.lld:pubmed
pubmed-article:16038613pubmed:affiliationDepartment of Biotechnology, Biological Faculty, Ogarev Mordovian State University, Bolshevistskaya ul. 68, 430000 Saransk, Russia. biotech@moris.rulld:pubmed
pubmed-article:16038613pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:16038613pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed