| pubmed-article:16012789 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:16012789 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:16012789 | lifeskim:mentions | umls-concept:C0281603 | lld:lifeskim |
| pubmed-article:16012789 | lifeskim:mentions | umls-concept:C1511636 | lld:lifeskim |
| pubmed-article:16012789 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:16012789 | lifeskim:mentions | umls-concept:C1709450 | lld:lifeskim |
| pubmed-article:16012789 | lifeskim:mentions | umls-concept:C2348628 | lld:lifeskim |
| pubmed-article:16012789 | lifeskim:mentions | umls-concept:C0950721 | lld:lifeskim |
| pubmed-article:16012789 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:16012789 | pubmed:dateCreated | 2005-7-13 | lld:pubmed |
| pubmed-article:16012789 | pubmed:abstractText | Flavopiridol and UCN-01 are two novel protein kinase inhibitors with diverse cellular effects that may complement each other with regards to induction of apoptosis. HeLa cells engineered to overexpress human survivin (HeLa-S) were at least approximately 4.8-fold resistant to UCN-01 relative to proliferation observed in control HeLa cells (HeLa-V). Flavopiridol cytotoxicity as measured using the MTT assay was unaffected in HeLa-S cells when compared with HeLa-V cells. Similarly, simultaneous treatment of HeLa-V cells with flavopiridol and UCN-01 for 72 hours did not result in synergistic inhibition of proliferation; however, in HeLa-S cells, this combination resulted in synergistic inhibition of cell proliferation. Flavopiridol and UCN-01 augmented apoptosis in HeLa-S cells (as compared with HeLa-V cells) as measured by caspase-3 cellular activity assay, DNA fragmentation and PARP cleavage by western blot. In HeLa-V and -S cells, combination treatment resulted in caspase-8 cleavage. Caspase-9 was expressed in HeLa-V cells; however, there was a marked reduction of caspase-9 content in HeLa-S cells only. Combination treatment resulted in a significant reduction in survivin abundance in HeLa-S and SKBR3-UR cells, but not in their respective parental lines. The synergy of Flavopiridol and UCN-01 are selectively toxic to survivin-overexpressing cell lines and the mechanism of toxicity involves caspase-dependent cell death. | lld:pubmed |
| pubmed-article:16012789 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16012789 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16012789 | pubmed:language | eng | lld:pubmed |
| pubmed-article:16012789 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16012789 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:16012789 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16012789 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16012789 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16012789 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16012789 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16012789 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16012789 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16012789 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16012789 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16012789 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16012789 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16012789 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16012789 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16012789 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16012789 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16012789 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16012789 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16012789 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:16012789 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:16012789 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:16012789 | pubmed:issn | 0167-6997 | lld:pubmed |
| pubmed-article:16012789 | pubmed:author | pubmed-author:ManiSridharS | lld:pubmed |
| pubmed-article:16012789 | pubmed:author | pubmed-author:D'AmicoMarkM | lld:pubmed |
| pubmed-article:16012789 | pubmed:author | pubmed-author:AlbaneseChris... | lld:pubmed |
| pubmed-article:16012789 | pubmed:author | pubmed-author:PestellRichar... | lld:pubmed |
| pubmed-article:16012789 | pubmed:author | pubmed-author:WangChenguang... | lld:pubmed |
| pubmed-article:16012789 | pubmed:author | pubmed-author:WuKongmingK | lld:pubmed |
| pubmed-article:16012789 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:16012789 | pubmed:volume | 23 | lld:pubmed |
| pubmed-article:16012789 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:16012789 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:16012789 | pubmed:pagination | 299-309 | lld:pubmed |
| pubmed-article:16012789 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:16012789 | pubmed:meshHeading | pubmed-meshheading:16012789... | lld:pubmed |
| pubmed-article:16012789 | pubmed:meshHeading | pubmed-meshheading:16012789... | lld:pubmed |
| pubmed-article:16012789 | pubmed:meshHeading | pubmed-meshheading:16012789... | lld:pubmed |
| pubmed-article:16012789 | pubmed:meshHeading | pubmed-meshheading:16012789... | lld:pubmed |
| pubmed-article:16012789 | pubmed:meshHeading | pubmed-meshheading:16012789... | lld:pubmed |
| pubmed-article:16012789 | pubmed:meshHeading | pubmed-meshheading:16012789... | lld:pubmed |
| pubmed-article:16012789 | pubmed:meshHeading | pubmed-meshheading:16012789... | lld:pubmed |
| pubmed-article:16012789 | pubmed:meshHeading | pubmed-meshheading:16012789... | lld:pubmed |
| pubmed-article:16012789 | pubmed:meshHeading | pubmed-meshheading:16012789... | lld:pubmed |
| pubmed-article:16012789 | pubmed:meshHeading | pubmed-meshheading:16012789... | lld:pubmed |
| pubmed-article:16012789 | pubmed:meshHeading | pubmed-meshheading:16012789... | lld:pubmed |
| pubmed-article:16012789 | pubmed:meshHeading | pubmed-meshheading:16012789... | lld:pubmed |
| pubmed-article:16012789 | pubmed:meshHeading | pubmed-meshheading:16012789... | lld:pubmed |
| pubmed-article:16012789 | pubmed:meshHeading | pubmed-meshheading:16012789... | lld:pubmed |
| pubmed-article:16012789 | pubmed:meshHeading | pubmed-meshheading:16012789... | lld:pubmed |
| pubmed-article:16012789 | pubmed:meshHeading | pubmed-meshheading:16012789... | lld:pubmed |
| pubmed-article:16012789 | pubmed:meshHeading | pubmed-meshheading:16012789... | lld:pubmed |
| pubmed-article:16012789 | pubmed:meshHeading | pubmed-meshheading:16012789... | lld:pubmed |
| pubmed-article:16012789 | pubmed:meshHeading | pubmed-meshheading:16012789... | lld:pubmed |
| pubmed-article:16012789 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:16012789 | pubmed:articleTitle | A study of cytotoxic synergy of UCN-01 and flavopiridol in syngeneic pair of cell lines. | lld:pubmed |
| pubmed-article:16012789 | pubmed:affiliation | Albert Einstein Comprehensive Cancer Center, Department of Medicine, Albert Einstein College of Medicine, Chanin 302, 1300 Morris Park Avenue, Bronx, NY 10461, USA. | lld:pubmed |
| pubmed-article:16012789 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:16012789 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:16012789 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:16012789 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:16012789 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
