| pubmed-article:15973420 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15973420 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:15973420 | lifeskim:mentions | umls-concept:C1171362 | lld:lifeskim |
| pubmed-article:15973420 | lifeskim:mentions | umls-concept:C1749467 | lld:lifeskim |
| pubmed-article:15973420 | lifeskim:mentions | umls-concept:C1947989 | lld:lifeskim |
| pubmed-article:15973420 | lifeskim:mentions | umls-concept:C0016107 | lld:lifeskim |
| pubmed-article:15973420 | lifeskim:mentions | umls-concept:C0422392 | lld:lifeskim |
| pubmed-article:15973420 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:15973420 | pubmed:dateCreated | 2005-6-23 | lld:pubmed |
| pubmed-article:15973420 | pubmed:abstractText | The implementation of efficient technologies for the production of recombinant mammalian proteins remains an outstanding challenge in many structural and functional genomics programs. We have developed a new method for rapid identification of soluble protein expression in E. coli, based on a separation of soluble protein from inclusion bodies by a filtration step at the colony level. The colony filtration (CoFi) blot is very well suited to screen libraries, and in the present work we used it to screen a deletion mutagenesis library. | lld:pubmed |
| pubmed-article:15973420 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15973420 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15973420 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15973420 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15973420 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15973420 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:15973420 | pubmed:issn | 1548-7091 | lld:pubmed |
| pubmed-article:15973420 | pubmed:author | pubmed-author:NordlundPärP | lld:pubmed |
| pubmed-article:15973420 | pubmed:author | pubmed-author:EkbergMonicaM | lld:pubmed |
| pubmed-article:15973420 | pubmed:author | pubmed-author:DahlrothSue-L... | lld:pubmed |
| pubmed-article:15973420 | pubmed:author | pubmed-author:CornvikTobias... | lld:pubmed |
| pubmed-article:15973420 | pubmed:author | pubmed-author:MagnusdottirA... | lld:pubmed |
| pubmed-article:15973420 | pubmed:author | pubmed-author:HermanMaria... | lld:pubmed |
| pubmed-article:15973420 | pubmed:author | pubmed-author:KnaustRosemar... | lld:pubmed |
| pubmed-article:15973420 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15973420 | pubmed:volume | 2 | lld:pubmed |
| pubmed-article:15973420 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15973420 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15973420 | pubmed:pagination | 507-9 | lld:pubmed |
| pubmed-article:15973420 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:15973420 | pubmed:meshHeading | pubmed-meshheading:15973420... | lld:pubmed |
| pubmed-article:15973420 | pubmed:meshHeading | pubmed-meshheading:15973420... | lld:pubmed |
| pubmed-article:15973420 | pubmed:meshHeading | pubmed-meshheading:15973420... | lld:pubmed |
| pubmed-article:15973420 | pubmed:meshHeading | pubmed-meshheading:15973420... | lld:pubmed |
| pubmed-article:15973420 | pubmed:meshHeading | pubmed-meshheading:15973420... | lld:pubmed |
| pubmed-article:15973420 | pubmed:meshHeading | pubmed-meshheading:15973420... | lld:pubmed |
| pubmed-article:15973420 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15973420 | pubmed:articleTitle | Colony filtration blot: a new screening method for soluble protein expression in Escherichia coli. | lld:pubmed |
| pubmed-article:15973420 | pubmed:affiliation | Department of Biochemistry and Biophysics, Stockholm University, S-106 91 Stockholm, Sweden. | lld:pubmed |
| pubmed-article:15973420 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15973420 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:15973420 | pubmed:publicationType | Evaluation Studies | lld:pubmed |
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