| pubmed-article:15881228 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15881228 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:15881228 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:15881228 | lifeskim:mentions | umls-concept:C0085828 | lld:lifeskim |
| pubmed-article:15881228 | lifeskim:mentions | umls-concept:C0567416 | lld:lifeskim |
| pubmed-article:15881228 | lifeskim:mentions | umls-concept:C0037083 | lld:lifeskim |
| pubmed-article:15881228 | lifeskim:mentions | umls-concept:C0007745 | lld:lifeskim |
| pubmed-article:15881228 | lifeskim:mentions | umls-concept:C1710082 | lld:lifeskim |
| pubmed-article:15881228 | lifeskim:mentions | umls-concept:C1551336 | lld:lifeskim |
| pubmed-article:15881228 | lifeskim:mentions | umls-concept:C0074992 | lld:lifeskim |
| pubmed-article:15881228 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:15881228 | pubmed:dateCreated | 2005-5-9 | lld:pubmed |
| pubmed-article:15881228 | pubmed:abstractText | We previously demonstrated that tumor necrosis factor (TNF)-alpha stimulated the production of activation protein (AP)-1, a transcriptional factor, in mouse osteoblastic MC3T3-E1 cells. Recent studies have shown the importance of ceramide and its metabolites as signal molecules for TNF-alpha-induced gene expression in several cell types. Therefore, our interest was to investigate whether sphingosine metabolites are involved in TNF-alpha-induced signaling in MC3T3-E1 cells. DL-threo-1-phenyl-2-hexadecanoyl-amino-3-pyrrolidino-1-propanol (PPPP), which causes accumulation of intracellular ceramide, stimulated the TNF-alpha-induced expression of the c-fos and c-jun genes. Gel shift assay clearly showed that PPPP increased the cytokine-induced specific binding of nuclear proteins to the 12-tetra-decanoyl phorbol 13-acetate-responsive element (TRE), a consensus sequence for AP-1. In addition, cell-permeable ceramide (N-acetylsphingosine, N-hexanoylsphingosine or N-octanoylsphingosine) stimulated expression of the c-fos and c-jun genes and nuclear protein binding to TRE. Interestingly, DL-threo-dihydrosphingosine (DHS), an inhibitor of sphingosine kinase, clearly blocked the ceramide analogue-induced stimulation. Sphingosine 1-phosphate (SPP) actually induced expression of these oncogenes and activated AP-1. Although TNF-alpha stimulated the AP-1-mediated expression of the monocyte chemoattractant JE/MCP-1, this stimulation was inhibited by DHS. SPP also stimulated JE/MCP-1 gene expression. The present study thus suggests that SPP acts as a signal molecule in ceramide-dependent signal transduction in TNF-alpha-induced AP-1 in osteoblastic MC3T3-E1 cells. | lld:pubmed |
| pubmed-article:15881228 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15881228 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15881228 | pubmed:citationSubset | D | lld:pubmed |
| pubmed-article:15881228 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15881228 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15881228 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15881228 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15881228 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15881228 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15881228 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15881228 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15881228 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15881228 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15881228 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15881228 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15881228 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15881228 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15881228 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15881228 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15881228 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15881228 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:15881228 | pubmed:issn | 1343-4934 | lld:pubmed |
| pubmed-article:15881228 | pubmed:author | pubmed-author:TakeshitaAkir... | lld:pubmed |
| pubmed-article:15881228 | pubmed:author | pubmed-author:MatsumotoKenK | lld:pubmed |
| pubmed-article:15881228 | pubmed:author | pubmed-author:KanegaeHaruhi... | lld:pubmed |
| pubmed-article:15881228 | pubmed:author | pubmed-author:SudaYokoY | lld:pubmed |
| pubmed-article:15881228 | pubmed:author | pubmed-author:TanakaSusumuS | lld:pubmed |
| pubmed-article:15881228 | pubmed:author | pubmed-author:NakabayashiYa... | lld:pubmed |
| pubmed-article:15881228 | pubmed:author | pubmed-author:ShinodaHiroyu... | lld:pubmed |
| pubmed-article:15881228 | pubmed:author | pubmed-author:YasuiToshikaz... | lld:pubmed |
| pubmed-article:15881228 | pubmed:author | pubmed-author:TakanoAkikoA | lld:pubmed |
| pubmed-article:15881228 | pubmed:author | pubmed-author:SuetsuguMayum... | lld:pubmed |
| pubmed-article:15881228 | pubmed:author | pubmed-author:MiyazawaKeiK | lld:pubmed |
| pubmed-article:15881228 | pubmed:author | pubmed-author:TanakaSonojiS | lld:pubmed |
| pubmed-article:15881228 | pubmed:author | pubmed-author:EndoHiromasaH | lld:pubmed |
| pubmed-article:15881228 | pubmed:author | pubmed-author:UeyamaYoshifu... | lld:pubmed |
| pubmed-article:15881228 | pubmed:author | pubmed-author:HanzawaAkikoA | lld:pubmed |
| pubmed-article:15881228 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15881228 | pubmed:volume | 47 | lld:pubmed |
| pubmed-article:15881228 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15881228 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15881228 | pubmed:pagination | 43-51 | lld:pubmed |
| pubmed-article:15881228 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:15881228 | pubmed:meshHeading | pubmed-meshheading:15881228... | lld:pubmed |
| pubmed-article:15881228 | pubmed:meshHeading | pubmed-meshheading:15881228... | lld:pubmed |
| pubmed-article:15881228 | pubmed:meshHeading | pubmed-meshheading:15881228... | lld:pubmed |
| pubmed-article:15881228 | pubmed:meshHeading | pubmed-meshheading:15881228... | lld:pubmed |
| pubmed-article:15881228 | pubmed:meshHeading | pubmed-meshheading:15881228... | lld:pubmed |
| pubmed-article:15881228 | pubmed:meshHeading | pubmed-meshheading:15881228... | lld:pubmed |
| pubmed-article:15881228 | pubmed:meshHeading | pubmed-meshheading:15881228... | lld:pubmed |
| pubmed-article:15881228 | pubmed:meshHeading | pubmed-meshheading:15881228... | lld:pubmed |
| pubmed-article:15881228 | pubmed:meshHeading | pubmed-meshheading:15881228... | lld:pubmed |
| pubmed-article:15881228 | pubmed:meshHeading | pubmed-meshheading:15881228... | lld:pubmed |
| pubmed-article:15881228 | pubmed:meshHeading | pubmed-meshheading:15881228... | lld:pubmed |
| pubmed-article:15881228 | pubmed:meshHeading | pubmed-meshheading:15881228... | lld:pubmed |
| pubmed-article:15881228 | pubmed:meshHeading | pubmed-meshheading:15881228... | lld:pubmed |
| pubmed-article:15881228 | pubmed:meshHeading | pubmed-meshheading:15881228... | lld:pubmed |
| pubmed-article:15881228 | pubmed:meshHeading | pubmed-meshheading:15881228... | lld:pubmed |
| pubmed-article:15881228 | pubmed:meshHeading | pubmed-meshheading:15881228... | lld:pubmed |
| pubmed-article:15881228 | pubmed:meshHeading | pubmed-meshheading:15881228... | lld:pubmed |
| pubmed-article:15881228 | pubmed:meshHeading | pubmed-meshheading:15881228... | lld:pubmed |
| pubmed-article:15881228 | pubmed:meshHeading | pubmed-meshheading:15881228... | lld:pubmed |
| pubmed-article:15881228 | pubmed:meshHeading | pubmed-meshheading:15881228... | lld:pubmed |
| pubmed-article:15881228 | pubmed:meshHeading | pubmed-meshheading:15881228... | lld:pubmed |
| pubmed-article:15881228 | pubmed:meshHeading | pubmed-meshheading:15881228... | lld:pubmed |
| pubmed-article:15881228 | pubmed:meshHeading | pubmed-meshheading:15881228... | lld:pubmed |
| pubmed-article:15881228 | pubmed:meshHeading | pubmed-meshheading:15881228... | lld:pubmed |
| pubmed-article:15881228 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15881228 | pubmed:articleTitle | Sphingosine 1-phosphate acts as a signal molecule in ceramide signal transduction of TNF-alpha-induced activator protein-1 in osteoblastic cell line MC3T3-E1 cells. | lld:pubmed |
| pubmed-article:15881228 | pubmed:affiliation | Department of Oral Health and Preventive Dentistry, Meikai University School of Dentistry, Keyakidai, Sakado-city, Saitama 350-0283, Japan. takesita@dent.meikai.ac.jp | lld:pubmed |
| pubmed-article:15881228 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15881228 | lld:pubmed |
