| pubmed-article:15817574 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15817574 | lifeskim:mentions | umls-concept:C0282560 | lld:lifeskim |
| pubmed-article:15817574 | lifeskim:mentions | umls-concept:C0012860 | lld:lifeskim |
| pubmed-article:15817574 | lifeskim:mentions | umls-concept:C0242184 | lld:lifeskim |
| pubmed-article:15817574 | lifeskim:mentions | umls-concept:C0751980 | lld:lifeskim |
| pubmed-article:15817574 | lifeskim:mentions | umls-concept:C0220825 | lld:lifeskim |
| pubmed-article:15817574 | lifeskim:mentions | umls-concept:C0450442 | lld:lifeskim |
| pubmed-article:15817574 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:15817574 | lifeskim:mentions | umls-concept:C0072863 | lld:lifeskim |
| pubmed-article:15817574 | lifeskim:mentions | umls-concept:C1527240 | lld:lifeskim |
| pubmed-article:15817574 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:15817574 | pubmed:dateCreated | 2005-5-11 | lld:pubmed |
| pubmed-article:15817574 | pubmed:abstractText | The DNA damage induced by 7-chloro-3-[[(N,N-dimethylamino)propyl]amino]-2-quinoxalinecarbonitrile 1,4-di-N-oxide hydrochloride (Q-85 HCl) in Caco-2 cells under hypoxic and well-oxygenated conditions has been studied by using the comet assay. This compound has shown a good in vitro profile of high selective toxicity in hypoxia, but its mechanism of action is unknown. The DNA damage has been evaluated by performing the comet assay after a 2-h treatment with Q-85 HCl (0.1, 0.2, 0.4 microM in hypoxia; 20, 40 microM in well-oxygenated conditions). The number of cells in apoptosis has also been assessed by flow cytometry analysis of Annexin V-FITC staining. The capability of the cells to repair the DNA damage and the proliferation rate was evaluated at different times after the treatment (24-168 h). Under hypoxic conditions, a clear dose-dependent increase in the number of nuclei with a comet was observed (comet score: 132 +/- 13, 343 +/- 30 and 399 +/- 1; control comet score: 42 +/- 14). Under well-oxygenated conditions, the number of nuclei with comet increased significantly with respect to the control (comet score: 273 +/- 14 and 312 +/- 9; control comet score: 27 +/- 4). Cells in apoptosis were not detected by the comet assay nor by flow cytometry. The recovery from DNA damage was time- and concentration-dependent in hypoxia (cells treated with the highest concentration still showed DNA damage after 72 h) and rather time-dependent in well-oxygenated conditions (DNA was completely repaired after 24 h). In conclusion, Q-85 HCl acts by DNA damage and not only the reduced intermediate is genotoxic but also some other derivatives and Q-85 HCl itself may be acting. | lld:pubmed |
| pubmed-article:15817574 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15817574 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15817574 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15817574 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15817574 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15817574 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15817574 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15817574 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15817574 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15817574 | pubmed:month | May | lld:pubmed |
| pubmed-article:15817574 | pubmed:issn | 0267-8357 | lld:pubmed |
| pubmed-article:15817574 | pubmed:author | pubmed-author:CascanteMarta... | lld:pubmed |
| pubmed-article:15817574 | pubmed:author | pubmed-author:CreppyEdmond... | lld:pubmed |
| pubmed-article:15817574 | pubmed:author | pubmed-author:AzquetaAmaiaA | lld:pubmed |
| pubmed-article:15817574 | pubmed:author | pubmed-author:López de... | lld:pubmed |
| pubmed-article:15817574 | pubmed:author | pubmed-author:PachónGiselaG | lld:pubmed |
| pubmed-article:15817574 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15817574 | pubmed:volume | 20 | lld:pubmed |
| pubmed-article:15817574 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15817574 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15817574 | pubmed:pagination | 165-71 | lld:pubmed |
| pubmed-article:15817574 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:15817574 | pubmed:meshHeading | pubmed-meshheading:15817574... | lld:pubmed |
| pubmed-article:15817574 | pubmed:meshHeading | pubmed-meshheading:15817574... | lld:pubmed |
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| pubmed-article:15817574 | pubmed:meshHeading | pubmed-meshheading:15817574... | lld:pubmed |
| pubmed-article:15817574 | pubmed:meshHeading | pubmed-meshheading:15817574... | lld:pubmed |
| pubmed-article:15817574 | pubmed:meshHeading | pubmed-meshheading:15817574... | lld:pubmed |
| pubmed-article:15817574 | pubmed:meshHeading | pubmed-meshheading:15817574... | lld:pubmed |
| pubmed-article:15817574 | pubmed:meshHeading | pubmed-meshheading:15817574... | lld:pubmed |
| pubmed-article:15817574 | pubmed:meshHeading | pubmed-meshheading:15817574... | lld:pubmed |
| pubmed-article:15817574 | pubmed:meshHeading | pubmed-meshheading:15817574... | lld:pubmed |
| pubmed-article:15817574 | pubmed:meshHeading | pubmed-meshheading:15817574... | lld:pubmed |
| pubmed-article:15817574 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15817574 | pubmed:articleTitle | DNA damage induced by a quinoxaline 1,4-di-N-oxide derivative (hypoxic selective agent) in Caco-2 cells evaluated by the comet assay. | lld:pubmed |
| pubmed-article:15817574 | pubmed:affiliation | Centro de Investigación en Farmacobiología Aplicada, University of Navarra, C/Irunlarrea 1, 31008 Pamplona, Spain. | lld:pubmed |
| pubmed-article:15817574 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15817574 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15817574 | lld:pubmed |
