| pubmed-article:15753259 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15753259 | lifeskim:mentions | umls-concept:C0019693 | lld:lifeskim |
| pubmed-article:15753259 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:15753259 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:15753259 | lifeskim:mentions | umls-concept:C0003315 | lld:lifeskim |
| pubmed-article:15753259 | lifeskim:mentions | umls-concept:C0011306 | lld:lifeskim |
| pubmed-article:15753259 | lifeskim:mentions | umls-concept:C0007613 | lld:lifeskim |
| pubmed-article:15753259 | lifeskim:mentions | umls-concept:C0221099 | lld:lifeskim |
| pubmed-article:15753259 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:15753259 | pubmed:dateCreated | 2005-3-8 | lld:pubmed |
| pubmed-article:15753259 | pubmed:abstractText | We generated human dendritic cell (DC) hybridoma cell lines by fusing HGPRT-deficient promonocytic U937 cells with immature DCs obtained by culturing peripheral blood monocytes with interleukin-4 (IL-4; 1,000 U/ml) and granulocyte-macrophage colony-stimulating factor (100 U/ml) for 7 days and mature DCs by treatment with tumor necrosis factor alpha (12.5 microg/ml) for 3 days. Only one fusion with immature DCs was successful and yielded four cell lines--HB-1, HB-2, HB-3, and HB-9--with an overall fusion efficiency of 0.0015%. The cell lines were stable in long-term culture, displayed morphological features typical of DCs, and expressed distinct class I and class II molecules not present on U937 (A*031012, B*51011, Cw*0701, DRB3*01011 52, and DR5*01011). A representative cell line, HB-2, that expressed DC markers including CD83, CD80 and CD86 could be induced to produce IL-12 through CD40 stimulation. After human immunodeficiency virus (HIV) infection, there was impairment of antigen-presenting cell (APC) function, which was manifested by an inability to stimulate allogeneic T-cell responses. There was no change in expression of major histocompatibility complex class I and class II antigens, CD83, CD40, CD4, CD11c, CD80, CD86, CD54, and CD58, or IL-12 production in the HIV-infected HB-2 cells. The HIV-infected HB-2 cells induced T-cell apoptosis in the cocultures. T-cell proliferation could be partially restored by using ddI, indinivir, and blocking anti-gp120 and anti-IL-10 antibodies. Our data suggest that there are multiple mechanisms that DCs use to inhibit T-cell responses in HIV-infected patients. The HB-2 cell line could be a useful model system to study APC function in HIV-infected DCs. | lld:pubmed |
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| pubmed-article:15753259 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15753259 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15753259 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15753259 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15753259 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15753259 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:15753259 | pubmed:issn | 1071-412X | lld:pubmed |
| pubmed-article:15753259 | pubmed:author | pubmed-author:SperberKirkK | lld:pubmed |
| pubmed-article:15753259 | pubmed:author | pubmed-author:BeuriaPrartha... | lld:pubmed |
| pubmed-article:15753259 | pubmed:author | pubmed-author:ChenHouchuH | lld:pubmed |
| pubmed-article:15753259 | pubmed:author | pubmed-author:TimoneyMichae... | lld:pubmed |
| pubmed-article:15753259 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15753259 | pubmed:volume | 12 | lld:pubmed |
| pubmed-article:15753259 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15753259 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15753259 | pubmed:pagination | 453-64 | lld:pubmed |
| pubmed-article:15753259 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:15753259 | pubmed:meshHeading | pubmed-meshheading:15753259... | lld:pubmed |
| pubmed-article:15753259 | pubmed:meshHeading | pubmed-meshheading:15753259... | lld:pubmed |
| pubmed-article:15753259 | pubmed:meshHeading | pubmed-meshheading:15753259... | lld:pubmed |
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| pubmed-article:15753259 | pubmed:meshHeading | pubmed-meshheading:15753259... | lld:pubmed |
| pubmed-article:15753259 | pubmed:meshHeading | pubmed-meshheading:15753259... | lld:pubmed |
| pubmed-article:15753259 | pubmed:meshHeading | pubmed-meshheading:15753259... | lld:pubmed |
| pubmed-article:15753259 | pubmed:meshHeading | pubmed-meshheading:15753259... | lld:pubmed |
| pubmed-article:15753259 | pubmed:meshHeading | pubmed-meshheading:15753259... | lld:pubmed |
| pubmed-article:15753259 | pubmed:meshHeading | pubmed-meshheading:15753259... | lld:pubmed |
| pubmed-article:15753259 | pubmed:meshHeading | pubmed-meshheading:15753259... | lld:pubmed |
| pubmed-article:15753259 | pubmed:meshHeading | pubmed-meshheading:15753259... | lld:pubmed |
| pubmed-article:15753259 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15753259 | pubmed:articleTitle | Impaired accessory cell function in a human dendritic cell line after human immunodeficiency virus infection. | lld:pubmed |
| pubmed-article:15753259 | pubmed:affiliation | Division of Clinical Immunology, Box 1089, 1 Gustave Levy Place, Mount Sinai School of Medicine, New York, NY 10029, USA. | lld:pubmed |
| pubmed-article:15753259 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15753259 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
