To construct different conformations of a plasmid DNA/vector complex (pcDNA3.1/IFN-gamma-ASOR-PLL) and transfect cells of the hepatoma cell line BEL7402 to investigate the optimal conformation of the complex for improved expression efficiency in the target cell.
Department of Gastroenterology, the Second XiangYa Hospital, Central South University and Sheng Life Gene Drug Development Company, Changsha, Hunan Province, China.