| pubmed-article:15665477 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15665477 | lifeskim:mentions | umls-concept:C0600435 | lld:lifeskim |
| pubmed-article:15665477 | lifeskim:mentions | umls-concept:C0005223 | lld:lifeskim |
| pubmed-article:15665477 | lifeskim:mentions | umls-concept:C1521827 | lld:lifeskim |
| pubmed-article:15665477 | lifeskim:mentions | umls-concept:C0871161 | lld:lifeskim |
| pubmed-article:15665477 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:15665477 | pubmed:dateCreated | 2005-1-24 | lld:pubmed |
| pubmed-article:15665477 | pubmed:abstractText | Hyperthermostable beta-glucosidase from Pyrococcus furiosus was enclosed in gelatin gel by cross-linking with transglutaminase. Gelatin-immobilized beta-glucosidase was considerably more thermostable than the native enzyme. Lyophilized immobilisate was stored at 90 degrees C for 1 month without loss of activity. The immobilized beta-glucosidase catalyzed transglucosylation of 5-phenylpentanol with 10.0 equivalent of cellobiose at pH 5.0 and 70 degrees C for 12 h to afford 5-phenylpentyl beta-D-glucopyranoside in 41% yield. The immobilized enzyme was more effective than the native one in transglucosylation. The gelatin-immobilized Pfu-beta-glucosidase recovered from the first run of the reaction was reusable on successive runs. | lld:pubmed |
| pubmed-article:15665477 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15665477 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15665477 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15665477 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15665477 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15665477 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15665477 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15665477 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15665477 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15665477 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15665477 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15665477 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15665477 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:15665477 | pubmed:issn | 0916-8451 | lld:pubmed |
| pubmed-article:15665477 | pubmed:author | pubmed-author:NagatomoHidet... | lld:pubmed |
| pubmed-article:15665477 | pubmed:author | pubmed-author:MatsushitaYoh... | lld:pubmed |
| pubmed-article:15665477 | pubmed:author | pubmed-author:SugamotoKazuh... | lld:pubmed |
| pubmed-article:15665477 | pubmed:author | pubmed-author:MatsuiTakanao... | lld:pubmed |
| pubmed-article:15665477 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15665477 | pubmed:volume | 69 | lld:pubmed |
| pubmed-article:15665477 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15665477 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15665477 | pubmed:pagination | 128-36 | lld:pubmed |
| pubmed-article:15665477 | pubmed:meshHeading | pubmed-meshheading:15665477... | lld:pubmed |
| pubmed-article:15665477 | pubmed:meshHeading | pubmed-meshheading:15665477... | lld:pubmed |
| pubmed-article:15665477 | pubmed:meshHeading | pubmed-meshheading:15665477... | lld:pubmed |
| pubmed-article:15665477 | pubmed:meshHeading | pubmed-meshheading:15665477... | lld:pubmed |
| pubmed-article:15665477 | pubmed:meshHeading | pubmed-meshheading:15665477... | lld:pubmed |
| pubmed-article:15665477 | pubmed:meshHeading | pubmed-meshheading:15665477... | lld:pubmed |
| pubmed-article:15665477 | pubmed:meshHeading | pubmed-meshheading:15665477... | lld:pubmed |
| pubmed-article:15665477 | pubmed:meshHeading | pubmed-meshheading:15665477... | lld:pubmed |
| pubmed-article:15665477 | pubmed:meshHeading | pubmed-meshheading:15665477... | lld:pubmed |
| pubmed-article:15665477 | pubmed:meshHeading | pubmed-meshheading:15665477... | lld:pubmed |
| pubmed-article:15665477 | pubmed:meshHeading | pubmed-meshheading:15665477... | lld:pubmed |
| pubmed-article:15665477 | pubmed:meshHeading | pubmed-meshheading:15665477... | lld:pubmed |
| pubmed-article:15665477 | pubmed:meshHeading | pubmed-meshheading:15665477... | lld:pubmed |
| pubmed-article:15665477 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15665477 | pubmed:articleTitle | Preparation and properties of gelatin-immobilized beta-glucosidase from Pyrococcus furiosus. | lld:pubmed |
| pubmed-article:15665477 | pubmed:affiliation | Faculty of Engineering, University of Miyazaki, Miyazaki, Japan. | lld:pubmed |
| pubmed-article:15665477 | pubmed:publicationType | Journal Article | lld:pubmed |
