| pubmed-article:15642781 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15642781 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:15642781 | lifeskim:mentions | umls-concept:C0007589 | lld:lifeskim |
| pubmed-article:15642781 | lifeskim:mentions | umls-concept:C2708700 | lld:lifeskim |
| pubmed-article:15642781 | lifeskim:mentions | umls-concept:C1511938 | lld:lifeskim |
| pubmed-article:15642781 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:15642781 | pubmed:dateCreated | 2005-1-11 | lld:pubmed |
| pubmed-article:15642781 | pubmed:abstractText | In order to study GH cell differentiation, we used the clonal cell lines called MtT/E and MtT/S cells, which were derived from a rat mammotrophic pituitary tumor. Although MtT/E cells are non-hormone-producing ones, Pit-1 protein is present in their nuclei, which suggests that MtT/E cells are progenitor cells of the Pit-1 cell lineage and have the potential to differentiate into hormone-producing cells. On the other hand, MtT/S cells produce GH; however, the responsiveness to GH-releasing hormone (GHRH) is weak and only a small number of secretory granules are present in their cytoplasm, which suggests that MtT/S cells are premature GH cells. In order to differentiate into GH cells from MtT/E cells as a progenitor cell, we examined several differentiation factors and found that retinoic acid (RA) induced the differentiation of MtT/E cells into GH-producing cells. RA-induced GH cells partially matured with the glucocorticoid treatment; however, the responsiveness to GHRH on GH secretion was incomplete. In order to elucidate the mechanism underlying full differentiation of GH cells, we used MtT/S cells. We treated MtT/S cells with glucocorticoid and found that they differentiated into mature GH cells with many secretory granules in their cytoplasm and they responded well to GHRH. These results suggested that MtT/E and MtT/S cells are progenitor or premature GH cells, and show different responses to differentiation factors. Our data also suggested that GH cells differentiate from their progenitor cells through multistep processes. | lld:pubmed |
| pubmed-article:15642781 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15642781 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15642781 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15642781 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15642781 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15642781 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15642781 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15642781 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15642781 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15642781 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15642781 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15642781 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15642781 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15642781 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15642781 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15642781 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:15642781 | pubmed:issn | 0022-0795 | lld:pubmed |
| pubmed-article:15642781 | pubmed:author | pubmed-author:InoueKK | lld:pubmed |
| pubmed-article:15642781 | pubmed:author | pubmed-author:YokoyamaKK | lld:pubmed |
| pubmed-article:15642781 | pubmed:author | pubmed-author:TomidaMM | lld:pubmed |
| pubmed-article:15642781 | pubmed:author | pubmed-author:MogiKK | lld:pubmed |
| pubmed-article:15642781 | pubmed:author | pubmed-author:TakakuMM | lld:pubmed |
| pubmed-article:15642781 | pubmed:author | pubmed-author:GodaHH | lld:pubmed |
| pubmed-article:15642781 | pubmed:author | pubmed-author:MogkAA | lld:pubmed |
| pubmed-article:15642781 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15642781 | pubmed:volume | 184 | lld:pubmed |
| pubmed-article:15642781 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15642781 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15642781 | pubmed:pagination | 41-50 | lld:pubmed |
| pubmed-article:15642781 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:15642781 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15642781 | pubmed:articleTitle | Multistep differentiation of GH-producing cells from their immature cells. | lld:pubmed |
| pubmed-article:15642781 | pubmed:affiliation | Department of Regulation Biology, Faculty of Science, Saitama University, 255 Shimo-Okubo, Sakura-ku, Saitama 338-8570, Japan. | lld:pubmed |
| pubmed-article:15642781 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15642781 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15642781 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15642781 | lld:pubmed |
