| pubmed-article:15631888 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15631888 | lifeskim:mentions | umls-concept:C0033085 | lld:lifeskim |
| pubmed-article:15631888 | lifeskim:mentions | umls-concept:C0184511 | lld:lifeskim |
| pubmed-article:15631888 | lifeskim:mentions | umls-concept:C0443286 | lld:lifeskim |
| pubmed-article:15631888 | lifeskim:mentions | umls-concept:C0026019 | lld:lifeskim |
| pubmed-article:15631888 | lifeskim:mentions | umls-concept:C1514468 | lld:lifeskim |
| pubmed-article:15631888 | lifeskim:mentions | umls-concept:C0049081 | lld:lifeskim |
| pubmed-article:15631888 | lifeskim:mentions | umls-concept:C0063186 | lld:lifeskim |
| pubmed-article:15631888 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:15631888 | pubmed:dateCreated | 2005-1-5 | lld:pubmed |
| pubmed-article:15631888 | pubmed:abstractText | In lineage tracing analysis, the beta-galactosidase (beta-gal) gene is a commonly used as a reporter gene because it is relatively stable and highly sensitive in histochemical detection using 5-bromo-4-chloro-3-indolyl-beta-d-galactoside (X-gal). Clear determination of the types and characteristics of labeled cells requires transmission electron microscopic (TEM) examination of their morphology. X-gal staining, which involves the precipitate formed by the reaction between beta-gal and X-gal, is usually recognized as a light blue or green reaction product on light microscopic (LM) examination. However, the standard protocol for TEM preparation weakens the intensity of or results in the loss of X-gal reaction product at the step of substitution of ethanol with Epon using propylene oxide. To solve this problem, we show that hydroxypropyl methacrylate achieves good preservation of X-gal reaction products. The protocol presented here appears to be useful for lineage determination by TEM of all types of X-gal-stained tissues. | lld:pubmed |
| pubmed-article:15631888 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15631888 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15631888 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15631888 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15631888 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15631888 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15631888 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15631888 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15631888 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15631888 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15631888 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:15631888 | pubmed:issn | 0304-3940 | lld:pubmed |
| pubmed-article:15631888 | pubmed:author | pubmed-author:TakebayashiHi... | lld:pubmed |
| pubmed-article:15631888 | pubmed:author | pubmed-author:IkenakaKazuhi... | lld:pubmed |
| pubmed-article:15631888 | pubmed:author | pubmed-author:OnoKatsuhikoK | lld:pubmed |
| pubmed-article:15631888 | pubmed:author | pubmed-author:DingLeiL | lld:pubmed |
| pubmed-article:15631888 | pubmed:author | pubmed-author:ShimizuKeijiK | lld:pubmed |
| pubmed-article:15631888 | pubmed:author | pubmed-author:MasahiraNorit... | lld:pubmed |
| pubmed-article:15631888 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15631888 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:15631888 | pubmed:volume | 374 | lld:pubmed |
| pubmed-article:15631888 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15631888 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15631888 | pubmed:pagination | 17-20 | lld:pubmed |
| pubmed-article:15631888 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:15631888 | pubmed:meshHeading | pubmed-meshheading:15631888... | lld:pubmed |
| pubmed-article:15631888 | pubmed:meshHeading | pubmed-meshheading:15631888... | lld:pubmed |
| pubmed-article:15631888 | pubmed:meshHeading | pubmed-meshheading:15631888... | lld:pubmed |
| pubmed-article:15631888 | pubmed:meshHeading | pubmed-meshheading:15631888... | lld:pubmed |
| pubmed-article:15631888 | pubmed:meshHeading | pubmed-meshheading:15631888... | lld:pubmed |
| pubmed-article:15631888 | pubmed:meshHeading | pubmed-meshheading:15631888... | lld:pubmed |
| pubmed-article:15631888 | pubmed:meshHeading | pubmed-meshheading:15631888... | lld:pubmed |
| pubmed-article:15631888 | pubmed:meshHeading | pubmed-meshheading:15631888... | lld:pubmed |
| pubmed-article:15631888 | pubmed:meshHeading | pubmed-meshheading:15631888... | lld:pubmed |
| pubmed-article:15631888 | pubmed:meshHeading | pubmed-meshheading:15631888... | lld:pubmed |
| pubmed-article:15631888 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15631888 | pubmed:articleTitle | Improved preservation of X-gal reaction product for electron microscopy using hydroxypropyl methacrylate. | lld:pubmed |
| pubmed-article:15631888 | pubmed:affiliation | Division of Neurobiology and Bioinformatics, National Institute for Physiological Sciences, Okazaki, Aichi 444-8787, Japan. | lld:pubmed |
| pubmed-article:15631888 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15631888 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:15631888 | pubmed:publicationType | Evaluation Studies | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15631888 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15631888 | lld:pubmed |
