| pubmed-article:1562594 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1562594 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:1562594 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:1562594 | lifeskim:mentions | umls-concept:C0205419 | lld:lifeskim |
| pubmed-article:1562594 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:1562594 | lifeskim:mentions | umls-concept:C0376315 | lld:lifeskim |
| pubmed-article:1562594 | lifeskim:mentions | umls-concept:C0051464 | lld:lifeskim |
| pubmed-article:1562594 | lifeskim:mentions | umls-concept:C1511636 | lld:lifeskim |
| pubmed-article:1562594 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:1562594 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:1562594 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:1562594 | pubmed:dateCreated | 1992-5-21 | lld:pubmed |
| pubmed-article:1562594 | pubmed:abstractText | An efficient Escherichia coli system for the production of a variant form of the cytotoxic protein alpha-sarcin (delta Ala 1) has been constructed. cDNA encoded alpha-sarcin lacking N-terminal alanine was ligated with the bla signal peptide sequence (the signal sequence of E. coli beta-lactamase localizes the protein in the periplasm) and was inserted into an inducible bacterial expression vector pKTN2-2. When the plasmid introduced into E. coli was expressed in the presence of IPTG, the recombinant product accumulated in the periplasmic space. The product was purified by Affi-Gel Blue followed by Bio-Rex 70 column chromatography. Recombinant alpha-sarcin (delta Ala 1) displayed the properties similar to those of authentic alpha-sarcin isolated from Aspergillus giganteus with respect to its molecular mass and enzymatic activity in ribosomal inactivation. The amount of alpha-sarcin variant produced in the system was estimated to be 1.2 mg/l of culture. | lld:pubmed |
| pubmed-article:1562594 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1562594 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1562594 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1562594 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1562594 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1562594 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1562594 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1562594 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1562594 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1562594 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1562594 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:1562594 | pubmed:issn | 0006-3002 | lld:pubmed |
| pubmed-article:1562594 | pubmed:author | pubmed-author:EndoYY | lld:pubmed |
| pubmed-article:1562594 | pubmed:author | pubmed-author:OkaTT | lld:pubmed |
| pubmed-article:1562594 | pubmed:author | pubmed-author:AoyamaYY | lld:pubmed |
| pubmed-article:1562594 | pubmed:author | pubmed-author:KatanoTT | lld:pubmed |
| pubmed-article:1562594 | pubmed:author | pubmed-author:NatoriYY | lld:pubmed |
| pubmed-article:1562594 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1562594 | pubmed:day | 24 | lld:pubmed |
| pubmed-article:1562594 | pubmed:volume | 1130 | lld:pubmed |
| pubmed-article:1562594 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1562594 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1562594 | pubmed:pagination | 182-8 | lld:pubmed |
| pubmed-article:1562594 | pubmed:dateRevised | 2006-5-1 | lld:pubmed |
| pubmed-article:1562594 | pubmed:meshHeading | pubmed-meshheading:1562594-... | lld:pubmed |
| pubmed-article:1562594 | pubmed:meshHeading | pubmed-meshheading:1562594-... | lld:pubmed |
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| pubmed-article:1562594 | pubmed:meshHeading | pubmed-meshheading:1562594-... | lld:pubmed |
| pubmed-article:1562594 | pubmed:meshHeading | pubmed-meshheading:1562594-... | lld:pubmed |
| pubmed-article:1562594 | pubmed:meshHeading | pubmed-meshheading:1562594-... | lld:pubmed |
| pubmed-article:1562594 | pubmed:meshHeading | pubmed-meshheading:1562594-... | lld:pubmed |
| pubmed-article:1562594 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1562594 | pubmed:articleTitle | An efficient expression system for a variant form of the cytotoxic protein alpha-sarcin in Escherichia coli. | lld:pubmed |
| pubmed-article:1562594 | pubmed:affiliation | Department of Nutritional Chemistry, School of Medicine, University of Tokushima, Japan. | lld:pubmed |
| pubmed-article:1562594 | pubmed:publicationType | Journal Article | lld:pubmed |
