| pubmed-article:15604695 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15604695 | lifeskim:mentions | umls-concept:C0085245 | lld:lifeskim |
| pubmed-article:15604695 | lifeskim:mentions | umls-concept:C0221102 | lld:lifeskim |
| pubmed-article:15604695 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:15604695 | lifeskim:mentions | umls-concept:C0005516 | lld:lifeskim |
| pubmed-article:15604695 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:15604695 | pubmed:dateCreated | 2004-12-17 | lld:pubmed |
| pubmed-article:15604695 | pubmed:abstractText | Cre recombinase gene from bacteriophage P1 was transiently expressed by a Potato Virus X (PVX)-based vector in transgenic lox -target Nicotiana benthamiana plants to remove the selectable marker gene. The target construct consisted of two directly oriented lox sites flanking a bar gene located between a gfp coding region and an upstream CaMV 35S promoter. The Cre-mediated excision of intervening sequence placed the gfp coding region under the transcriptional control of the CaMV 35S promoter. GFP activity was observed in PVX-Cre systemically infected leaves, regenerants from PVX-Cre infected explants and T1 progeny of these regenerants. PVX-Cre was removed efficiently from the regenerants by adding the nucleoside analogue ribavirin to the culture medium. Molecular data proved a correlation between gfp expression and precise site-specific excision of the bar gene in all examined transgenic lines. The frequency of recombination expressed as a percentage of regenerated plants exhibiting marker gene excision varied from 48% to 82%. These results demonstrate that a plant virus vector can be used efficiently to express cre recombinase in vivo providing an alternative method for the production of transgenic plants without marker genes. | lld:pubmed |
| pubmed-article:15604695 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15604695 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15604695 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15604695 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15604695 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15604695 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15604695 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15604695 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15604695 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15604695 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15604695 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15604695 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15604695 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15604695 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15604695 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:15604695 | pubmed:issn | 0167-4412 | lld:pubmed |
| pubmed-article:15604695 | pubmed:author | pubmed-author:SchiemannJJ | lld:pubmed |
| pubmed-article:15604695 | pubmed:author | pubmed-author:JüttnerGG | lld:pubmed |
| pubmed-article:15604695 | pubmed:author | pubmed-author:KopertekhLL | lld:pubmed |
| pubmed-article:15604695 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15604695 | pubmed:volume | 55 | lld:pubmed |
| pubmed-article:15604695 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15604695 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15604695 | pubmed:pagination | 491-500 | lld:pubmed |
| pubmed-article:15604695 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:15604695 | pubmed:meshHeading | pubmed-meshheading:15604695... | lld:pubmed |
| pubmed-article:15604695 | pubmed:meshHeading | pubmed-meshheading:15604695... | lld:pubmed |
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| pubmed-article:15604695 | pubmed:meshHeading | pubmed-meshheading:15604695... | lld:pubmed |
| pubmed-article:15604695 | pubmed:meshHeading | pubmed-meshheading:15604695... | lld:pubmed |
| pubmed-article:15604695 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15604695 | pubmed:articleTitle | PVX-Cre-mediated marker gene elimination from transgenic plants. | lld:pubmed |
| pubmed-article:15604695 | pubmed:affiliation | Federal Biological Research Centre for Agriculture and Forestry, Institute for Plant Virology, Microbiology and Biosafety, Messeweg 11-12, Braunschweig, Germany. | lld:pubmed |
| pubmed-article:15604695 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15604695 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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