| pubmed-article:15592646 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15592646 | lifeskim:mentions | umls-concept:C0037380 | lld:lifeskim |
| pubmed-article:15592646 | lifeskim:mentions | umls-concept:C0206313 | lld:lifeskim |
| pubmed-article:15592646 | lifeskim:mentions | umls-concept:C0014442 | lld:lifeskim |
| pubmed-article:15592646 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:15592646 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:15592646 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:15592646 | pubmed:dateCreated | 2004-12-13 | lld:pubmed |
| pubmed-article:15592646 | pubmed:abstractText | A thrombin-like serine protease, jararassin-I, was isolated from the venom of Bothrops jararaca. The protein was obtained in high yield and purity by a single chromatographic step using the affinity resin Benzamidine-Sepharose CL-6B. SDS-PAGE and dynamic light scattering analyses indicated that the molecular mass of the enzyme was about 30 kD. The enzyme possessed fibrinogenolytic and coagulant activities. The jararassin-I degraded the Bb chain of fibrinogen while the Aa chain and g chain were unchanged. Proteases inhibitors, PMSF and benzamidine inhibited the coagulant activity. These results showed jararassin-I is a serine protease similar to coagulating thrombin-like snake venom proteases, but it specifically cleaves Bb chain of bovine fibrinogen. Single crystals of enzyme were obtained (0.2 mm x 0.2 mm x 0.2 mm) and used for X-ray diffraction experiments. | lld:pubmed |
| pubmed-article:15592646 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15592646 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15592646 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15592646 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15592646 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15592646 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15592646 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15592646 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15592646 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:15592646 | pubmed:issn | 1672-9145 | lld:pubmed |
| pubmed-article:15592646 | pubmed:author | pubmed-author:SoaresAndreim... | lld:pubmed |
| pubmed-article:15592646 | pubmed:author | pubmed-author:ArniRaghuvir... | lld:pubmed |
| pubmed-article:15592646 | pubmed:author | pubmed-author:WatanabeLeand... | lld:pubmed |
| pubmed-article:15592646 | pubmed:author | pubmed-author:MarcussiSilva... | lld:pubmed |
| pubmed-article:15592646 | pubmed:author | pubmed-author:SampaioSuely... | lld:pubmed |
| pubmed-article:15592646 | pubmed:author | pubmed-author:VieiraDëora... | lld:pubmed |
| pubmed-article:15592646 | pubmed:author | pubmed-author:Sant'anaCarol... | lld:pubmed |
| pubmed-article:15592646 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15592646 | pubmed:volume | 36 | lld:pubmed |
| pubmed-article:15592646 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15592646 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15592646 | pubmed:pagination | 798-802 | lld:pubmed |
| pubmed-article:15592646 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:15592646 | pubmed:meshHeading | pubmed-meshheading:15592646... | lld:pubmed |
| pubmed-article:15592646 | pubmed:meshHeading | pubmed-meshheading:15592646... | lld:pubmed |
| pubmed-article:15592646 | pubmed:meshHeading | pubmed-meshheading:15592646... | lld:pubmed |
| pubmed-article:15592646 | pubmed:meshHeading | pubmed-meshheading:15592646... | lld:pubmed |
| pubmed-article:15592646 | pubmed:meshHeading | pubmed-meshheading:15592646... | lld:pubmed |
| pubmed-article:15592646 | pubmed:meshHeading | pubmed-meshheading:15592646... | lld:pubmed |
| pubmed-article:15592646 | pubmed:meshHeading | pubmed-meshheading:15592646... | lld:pubmed |
| pubmed-article:15592646 | pubmed:meshHeading | pubmed-meshheading:15592646... | lld:pubmed |
| pubmed-article:15592646 | pubmed:meshHeading | pubmed-meshheading:15592646... | lld:pubmed |
| pubmed-article:15592646 | pubmed:meshHeading | pubmed-meshheading:15592646... | lld:pubmed |
| pubmed-article:15592646 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15592646 | pubmed:articleTitle | Purification and characterization of jararassin-I, A thrombin-like enzyme from Bothrops jararaca snake venom. | lld:pubmed |
| pubmed-article:15592646 | pubmed:affiliation | Departamento de Física, Instituto de Biociências Exatas, Universidade Estadual Paulista, UNESP, São José do Rio Preto, SP, Brazil. | lld:pubmed |
| pubmed-article:15592646 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15592646 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
