| pubmed-article:15522917 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15522917 | lifeskim:mentions | umls-concept:C0318342 | lld:lifeskim |
| pubmed-article:15522917 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
| pubmed-article:15522917 | lifeskim:mentions | umls-concept:C0025252 | lld:lifeskim |
| pubmed-article:15522917 | lifeskim:mentions | umls-concept:C0009013 | lld:lifeskim |
| pubmed-article:15522917 | lifeskim:mentions | umls-concept:C0034790 | lld:lifeskim |
| pubmed-article:15522917 | lifeskim:mentions | umls-concept:C1171362 | lld:lifeskim |
| pubmed-article:15522917 | lifeskim:mentions | umls-concept:C1332714 | lld:lifeskim |
| pubmed-article:15522917 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:15522917 | lifeskim:mentions | umls-concept:C1515670 | lld:lifeskim |
| pubmed-article:15522917 | lifeskim:mentions | umls-concept:C0205164 | lld:lifeskim |
| pubmed-article:15522917 | lifeskim:mentions | umls-concept:C1710548 | lld:lifeskim |
| pubmed-article:15522917 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:15522917 | pubmed:dateCreated | 2005-1-27 | lld:pubmed |
| pubmed-article:15522917 | pubmed:abstractText | Major surface protein 2 (MSP2) of the bovine rickettsial pathogen Anaplasma marginale is an abundant, serologically immunodominant outer membrane protein. Immunodominance partially results from numerous CD4+ T cell epitopes in highly conserved amino and carboxy regions and the central hypervariable region of MSP2. However, in long-term cultures of lymphocytes stimulated with A. marginale, workshop cluster 1 (WC1)+ gammadelta T cells and CD4+ alphabeta T cells proliferated, leading to a predominance of gammadelta T cells. As gammadelta T cells proliferate in A. marginale-stimulated lymphocyte cultures, this study hypothesized that gammadelta T cells respond to the abundant, immunodominant MSP2. To test this hypothesis, gammadelta T cell clones were isolated from MSP2 vaccinates and assessed for antigen-specific proliferation and interferon-gamma secretion. Seven WC1+ gammadelta T cell clones responded to A. marginale and MSP2, and three of these proliferated to overlapping peptides from the conserved carboxy region. The gammadelta T cell response was not major histocompatibility complex-restricted, although it required antigen-presenting cells and was blocked by addition of antibody specific for the T cell receptor (TCR). Sequence analysis of TCR-gamma and -delta chains of peripheral blood lymphocytes identified two novel TCR-gamma chain constant (Cgamma) regions. It is important that all seven MSP2-specific gammadelta T cell clones used the same one of these novel Cgamma regions. The TCR complementarity-determining region 3 was less conserved than those of MSP2-specific CD4+ alphabeta T cell clones. Together, these data indicate that WC1+ gammadelta T cells recognize A. marginale MSP2 through the TCR and contribute to the immunodominant response to this protein. | lld:pubmed |
| pubmed-article:15522917 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15522917 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15522917 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15522917 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15522917 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15522917 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15522917 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15522917 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15522917 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15522917 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15522917 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:15522917 | pubmed:issn | 0741-5400 | lld:pubmed |
| pubmed-article:15522917 | pubmed:author | pubmed-author:PalmerGuy HGH | lld:pubmed |
| pubmed-article:15522917 | pubmed:author | pubmed-author:BrownWendy... | lld:pubmed |
| pubmed-article:15522917 | pubmed:author | pubmed-author:NorimineJunzo... | lld:pubmed |
| pubmed-article:15522917 | pubmed:author | pubmed-author:AbrahamsenMit... | lld:pubmed |
| pubmed-article:15522917 | pubmed:author | pubmed-author:LahmersKevin... | lld:pubmed |
| pubmed-article:15522917 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15522917 | pubmed:volume | 77 | lld:pubmed |
| pubmed-article:15522917 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15522917 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15522917 | pubmed:pagination | 199-208 | lld:pubmed |
| pubmed-article:15522917 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:15522917 | pubmed:meshHeading | pubmed-meshheading:15522917... | lld:pubmed |
| pubmed-article:15522917 | pubmed:meshHeading | pubmed-meshheading:15522917... | lld:pubmed |
| pubmed-article:15522917 | pubmed:meshHeading | pubmed-meshheading:15522917... | lld:pubmed |
| pubmed-article:15522917 | pubmed:meshHeading | pubmed-meshheading:15522917... | lld:pubmed |
| pubmed-article:15522917 | pubmed:meshHeading | pubmed-meshheading:15522917... | lld:pubmed |
| pubmed-article:15522917 | pubmed:meshHeading | pubmed-meshheading:15522917... | lld:pubmed |
| pubmed-article:15522917 | pubmed:meshHeading | pubmed-meshheading:15522917... | lld:pubmed |
| pubmed-article:15522917 | pubmed:meshHeading | pubmed-meshheading:15522917... | lld:pubmed |
| pubmed-article:15522917 | pubmed:meshHeading | pubmed-meshheading:15522917... | lld:pubmed |
| pubmed-article:15522917 | pubmed:meshHeading | pubmed-meshheading:15522917... | lld:pubmed |
| pubmed-article:15522917 | pubmed:meshHeading | pubmed-meshheading:15522917... | lld:pubmed |
| pubmed-article:15522917 | pubmed:meshHeading | pubmed-meshheading:15522917... | lld:pubmed |
| pubmed-article:15522917 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15522917 | pubmed:articleTitle | The CD4+ T cell immunodominant Anaplasma marginale major surface protein 2 stimulates gammadelta T cell clones that express unique T cell receptors. | lld:pubmed |
| pubmed-article:15522917 | pubmed:affiliation | Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USA. | lld:pubmed |
| pubmed-article:15522917 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15522917 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:15522917 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:15522917 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| entrez-gene:535300 | entrezgene:pubmed | pubmed-article:15522917 | lld:entrezgene |
| entrez-gene:781378 | entrezgene:pubmed | pubmed-article:15522917 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15522917 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15522917 | lld:pubmed |
