| pubmed-article:15451253 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15451253 | lifeskim:mentions | umls-concept:C0007452 | lld:lifeskim |
| pubmed-article:15451253 | lifeskim:mentions | umls-concept:C0013935 | lld:lifeskim |
| pubmed-article:15451253 | lifeskim:mentions | umls-concept:C0005712 | lld:lifeskim |
| pubmed-article:15451253 | lifeskim:mentions | umls-concept:C0015915 | lld:lifeskim |
| pubmed-article:15451253 | lifeskim:mentions | umls-concept:C1441547 | lld:lifeskim |
| pubmed-article:15451253 | lifeskim:mentions | umls-concept:C0162789 | lld:lifeskim |
| pubmed-article:15451253 | lifeskim:mentions | umls-concept:C0205171 | lld:lifeskim |
| pubmed-article:15451253 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:15451253 | pubmed:dateCreated | 2004-9-28 | lld:pubmed |
| pubmed-article:15451253 | pubmed:abstractText | Fluorescence in situ hybridization (FISH) is a sensitive technique for molecular diagnosis of chromosomes on single cells and can be applied to sex determination of embryos. The objective has been to develop an accurate and reliable bovine Y chromosome-specific DNA probe in order to sex biopsed blastomeres derived from IVF bovine embryos by FISH. Bovine Y chromosome-specific PCR product derived from BtY2 sequences was labeled with biotin-16-dUTP (BtY2-L1 probe), and FISH was performed on karyoplasts of biopsed blastomeres and matched demi-embryos. Our FISH signal was clearly detected in nuclei of blastomeres of male embryos. FISH analysis of bovine embryos gave high reliability (96%) between biopsied blastomeres and matched demi-embryos. These results indicated that the BtY2-L1 bovine Y chromosome-specific FISH probe was an effective probe for bovine embryo sexing, and the FISH technique of probe detection could improve the efficiency and reliability. | lld:pubmed |
| pubmed-article:15451253 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15451253 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15451253 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15451253 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15451253 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15451253 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:15451253 | pubmed:issn | 0093-691X | lld:pubmed |
| pubmed-article:15451253 | pubmed:author | pubmed-author:LeeJong HoJH | lld:pubmed |
| pubmed-article:15451253 | pubmed:author | pubmed-author:LeeSung-HakSH | lld:pubmed |
| pubmed-article:15451253 | pubmed:author | pubmed-author:ParkJoong... | lld:pubmed |
| pubmed-article:15451253 | pubmed:author | pubmed-author:ParkChang... | lld:pubmed |
| pubmed-article:15451253 | pubmed:author | pubmed-author:JinDong IlDI | lld:pubmed |
| pubmed-article:15451253 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15451253 | pubmed:volume | 62 | lld:pubmed |
| pubmed-article:15451253 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15451253 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15451253 | pubmed:pagination | 1452-8 | lld:pubmed |
| pubmed-article:15451253 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
| pubmed-article:15451253 | pubmed:meshHeading | pubmed-meshheading:15451253... | lld:pubmed |
| pubmed-article:15451253 | pubmed:meshHeading | pubmed-meshheading:15451253... | lld:pubmed |
| pubmed-article:15451253 | pubmed:meshHeading | pubmed-meshheading:15451253... | lld:pubmed |
| pubmed-article:15451253 | pubmed:meshHeading | pubmed-meshheading:15451253... | lld:pubmed |
| pubmed-article:15451253 | pubmed:meshHeading | pubmed-meshheading:15451253... | lld:pubmed |
| pubmed-article:15451253 | pubmed:meshHeading | pubmed-meshheading:15451253... | lld:pubmed |
| pubmed-article:15451253 | pubmed:meshHeading | pubmed-meshheading:15451253... | lld:pubmed |
| pubmed-article:15451253 | pubmed:meshHeading | pubmed-meshheading:15451253... | lld:pubmed |
| pubmed-article:15451253 | pubmed:meshHeading | pubmed-meshheading:15451253... | lld:pubmed |
| pubmed-article:15451253 | pubmed:meshHeading | pubmed-meshheading:15451253... | lld:pubmed |
| pubmed-article:15451253 | pubmed:meshHeading | pubmed-meshheading:15451253... | lld:pubmed |
| pubmed-article:15451253 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15451253 | pubmed:articleTitle | Sexing using single blastomere derived from IVF bovine embryos by fluorescence in situ hybridization (FISH). | lld:pubmed |
| pubmed-article:15451253 | pubmed:affiliation | Department of Animal Science and Technology, Seoul National University, Suwon City, South Korea. | lld:pubmed |
| pubmed-article:15451253 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15451253 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
