| pubmed-article:1544568 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1544568 | lifeskim:mentions | umls-concept:C0043393 | lld:lifeskim |
| pubmed-article:1544568 | lifeskim:mentions | umls-concept:C0282188 | lld:lifeskim |
| pubmed-article:1544568 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:1544568 | pubmed:dateCreated | 1992-4-13 | lld:pubmed |
| pubmed-article:1544568 | pubmed:abstractText | A set of four yeast shuttle vectors that incorporate sequences from the Saccharomyces cerevisiae 2 mu endogenous plasmid has been constructed. These yeast episomal plasmid (YEp)-type vectors (pRS420 series) differ only in their yeast selectable markers, HIS3, TRP1, LEU2 or URA3. The pRS420 plasmids are based on the backbone of a multifunctional phagemid, pBluescript II SK+, and share its useful properties for growth in Escherichia coli and manipulation in vitro. The pRS420 plasmids have a copy number of about 20 per cell, equivalent to that of YEp24. During non-selective yeast growth, pRS420 plasmids are lost through mitotic segregation at rates similar to other YEp vectors and yeast centromeric plasmid (YCp) vectors, in the range of 1.5-5% of progeny per doubling. The pRS420 series provides high-copy-number counterparts to the current pRS vectors [Sikorski and Hieter, Genetics 122 (1989) 19-27]. | lld:pubmed |
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| pubmed-article:1544568 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1544568 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1544568 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1544568 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1544568 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1544568 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1544568 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:1544568 | pubmed:issn | 0378-1119 | lld:pubmed |
| pubmed-article:1544568 | pubmed:author | pubmed-author:HieterPP | lld:pubmed |
| pubmed-article:1544568 | pubmed:author | pubmed-author:SikorskiR SRS | lld:pubmed |
| pubmed-article:1544568 | pubmed:author | pubmed-author:SheroJ HJH | lld:pubmed |
| pubmed-article:1544568 | pubmed:author | pubmed-author:ChristiansonT... | lld:pubmed |
| pubmed-article:1544568 | pubmed:author | pubmed-author:DanteMM | lld:pubmed |
| pubmed-article:1544568 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1544568 | pubmed:day | 2 | lld:pubmed |
| pubmed-article:1544568 | pubmed:volume | 110 | lld:pubmed |
| pubmed-article:1544568 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1544568 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1544568 | pubmed:pagination | 119-22 | lld:pubmed |
| pubmed-article:1544568 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:1544568 | pubmed:meshHeading | pubmed-meshheading:1544568-... | lld:pubmed |
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| pubmed-article:1544568 | pubmed:meshHeading | pubmed-meshheading:1544568-... | lld:pubmed |
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| pubmed-article:1544568 | pubmed:meshHeading | pubmed-meshheading:1544568-... | lld:pubmed |
| pubmed-article:1544568 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1544568 | pubmed:articleTitle | Multifunctional yeast high-copy-number shuttle vectors. | lld:pubmed |
| pubmed-article:1544568 | pubmed:affiliation | Department of Microbiology, Southern Illinois University, Carbondale 62901. | lld:pubmed |
| pubmed-article:1544568 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1544568 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1544568 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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