| pubmed-article:15386356 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15386356 | lifeskim:mentions | umls-concept:C0024109 | lld:lifeskim |
| pubmed-article:15386356 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:15386356 | lifeskim:mentions | umls-concept:C0014257 | lld:lifeskim |
| pubmed-article:15386356 | lifeskim:mentions | umls-concept:C0699790 | lld:lifeskim |
| pubmed-article:15386356 | lifeskim:mentions | umls-concept:C0001511 | lld:lifeskim |
| pubmed-article:15386356 | lifeskim:mentions | umls-concept:C1518174 | lld:lifeskim |
| pubmed-article:15386356 | lifeskim:mentions | umls-concept:C0079189 | lld:lifeskim |
| pubmed-article:15386356 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:15386356 | pubmed:dateCreated | 2004-10-21 | lld:pubmed |
| pubmed-article:15386356 | pubmed:abstractText | In this experimental study, the influence of surgery-induced proinflammatory cytokines on tumor recurrence in the lung was investigated. A reproducible human in vitro assay was developed to study the adhesion of HT29 colon carcinoma cells to monolayers of microvascular endothelial cells of the lung (HMVECs-L) or human umbilical venous endothelial cells (HUVECs). Preincubation of HMVECs-L with maximally active concentrations of IL-1beta and TNF-alpha, but not with IL-6, resulted in at least 250% adhesion compared to control adhesion (p <or= 0.01). The effect of IL-1beta and TNF-alpha was concentration- and time-dependent. Comparable results were found for HUVECs. Tumor cell adhesion was not increased after preincubation of HT29 with TNF-alpha. Enzyme immunoassays of cytokine-preincubated HUVECs and HMVECs-L showed concentration- and time-dependent upregulation of E-selectin, ICAM-1 and VCAM-1 expression. In addition, LFA-1 and VLA-4 were only expressed on HMVECs-L, creating more binding possibilities for HMVECs-L compared to HUVECs. Inhibition assays with anti-E-selectin monoclonal antibody significantly decreased tumor cell adhesion to HUVECs; however, it did not affect tumor cell adhesion to HMVECs-L. Furthermore, anti-ICAM-1 and anti-VCAM-1 antibodies did not affect adhesion. Our results prove IL-1beta and TNF-alpha promote tumor cell adhesion to HMVECs-L in vitro and may therefore account for enhanced tumor recurrence in the lung seen after major surgical trauma. The adhesion of HT29 to HUVEC is inhibitable by E-selectin antibodies, whereas the adhesion to HMVEC-L is not inhibitable by these antibodies. Probably not one but a complex of adhesion molecules is responsible for enhanced adhesion to HMVECs-L. | lld:pubmed |
| pubmed-article:15386356 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15386356 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15386356 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15386356 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:15386356 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15386356 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:15386356 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15386356 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15386356 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:15386356 | pubmed:issn | 0020-7136 | lld:pubmed |
| pubmed-article:15386356 | pubmed:author | pubmed-author:HoflandLeo... | lld:pubmed |
| pubmed-article:15386356 | pubmed:author | pubmed-author:JeekelJohanne... | lld:pubmed |
| pubmed-article:15386356 | pubmed:author | pubmed-author:van... | lld:pubmed |
| pubmed-article:15386356 | pubmed:author | pubmed-author:ten... | lld:pubmed |
| pubmed-article:15386356 | pubmed:author | pubmed-author:van... | lld:pubmed |
| pubmed-article:15386356 | pubmed:author | pubmed-author:van... | lld:pubmed |
| pubmed-article:15386356 | pubmed:copyrightInfo | (c) 2004 Wiley-Liss, Inc. | lld:pubmed |
| pubmed-article:15386356 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15386356 | pubmed:day | 20 | lld:pubmed |
| pubmed-article:15386356 | pubmed:volume | 112 | lld:pubmed |
| pubmed-article:15386356 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15386356 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15386356 | pubmed:pagination | 943-50 | lld:pubmed |
| pubmed-article:15386356 | pubmed:dateRevised | 2007-7-24 | lld:pubmed |
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| pubmed-article:15386356 | pubmed:meshHeading | pubmed-meshheading:15386356... | lld:pubmed |
| pubmed-article:15386356 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15386356 | pubmed:articleTitle | Influence of proinflammatory cytokines on the adhesion of human colon carcinoma cells to lung microvascular endothelium. | lld:pubmed |
| pubmed-article:15386356 | pubmed:affiliation | Department of Surgery, Erasmus Medical Center, Rotterdam, The Netherlands. | lld:pubmed |
| pubmed-article:15386356 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15386356 | pubmed:publicationType | In Vitro | lld:pubmed |
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