| pubmed-article:15349913 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15349913 | lifeskim:mentions | umls-concept:C0369332 | lld:lifeskim |
| pubmed-article:15349913 | lifeskim:mentions | umls-concept:C0030705 | lld:lifeskim |
| pubmed-article:15349913 | lifeskim:mentions | umls-concept:C0524909 | lld:lifeskim |
| pubmed-article:15349913 | lifeskim:mentions | umls-concept:C1709793 | lld:lifeskim |
| pubmed-article:15349913 | lifeskim:mentions | umls-concept:C0587267 | lld:lifeskim |
| pubmed-article:15349913 | lifeskim:mentions | umls-concept:C1282913 | lld:lifeskim |
| pubmed-article:15349913 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:15349913 | pubmed:dateCreated | 2004-9-6 | lld:pubmed |
| pubmed-article:15349913 | pubmed:abstractText | This study examined a signal amplification assay, the Invader assay, for the quantitation of hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) in liver biopsies and sera. DNA was extracted from liver biopsy and serum samples were collected from 16 hepatitis B e antigen (HBeAg)-positive and 36 antibody-to-HBeAg-positive (anti-HBe-positive) chronic hepatitis B patients. The amount of total HBV DNA and cccDNA was measured using the Invader assay. Anti-HBe-positive patients had lower median total intrahepatic HBV DNA (P < .001) and intrahepatic cccDNA levels (P = .001) than HBeAg-positive patients. Intrahepatic cccDNA correlated positively with the total intrahepatic HBV DNA (r = 0.950, P < .001). However, the proportion of intrahepatic HBV DNA in the form of cccDNA was inversely related to the amount of total intrahepatic HBV DNA (r = -0.822, P < .001). A small amount of cccDNA was detected in 39 of 52 (75%) serum samples. Anti-HBe-positive patients had lower median serum cccDNA levels than HBeAg-positive patients (P = .002). Serum HBV DNA correlated positively with intrahepatic total HBV DNA (r = 0.778, P < .001) and intrahepatic cccDNA (r = 0.481, P = .002). In conclusion, the Invader assay is a reliable assay for the quantitation of cccDNA. Serum and intrahepatic total HBV DNA and cccDNA levels become lower as the disease progresses from HBeAg-positive to anti-HBe-positive phase, with cccDNA becoming the predominant form of intrahepatic HBV DNA. | lld:pubmed |
| pubmed-article:15349913 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15349913 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15349913 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15349913 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15349913 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15349913 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15349913 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15349913 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:15349913 | pubmed:issn | 0270-9139 | lld:pubmed |
| pubmed-article:15349913 | pubmed:author | pubmed-author:YuenMan-FungM... | lld:pubmed |
| pubmed-article:15349913 | pubmed:author | pubmed-author:LaiChing-Lung... | lld:pubmed |
| pubmed-article:15349913 | pubmed:author | pubmed-author:HuiChee-KinCK | lld:pubmed |
| pubmed-article:15349913 | pubmed:author | pubmed-author:WongDanny... | lld:pubmed |
| pubmed-article:15349913 | pubmed:author | pubmed-author:HallJeffJ | lld:pubmed |
| pubmed-article:15349913 | pubmed:author | pubmed-author:SumSimon... | lld:pubmed |
| pubmed-article:15349913 | pubmed:author | pubmed-author:YuanHeJunH | lld:pubmed |
| pubmed-article:15349913 | pubmed:copyrightInfo | Copyright 2004 American Association for the Study of Liver Diseases | lld:pubmed |
| pubmed-article:15349913 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15349913 | pubmed:volume | 40 | lld:pubmed |
| pubmed-article:15349913 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15349913 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15349913 | pubmed:pagination | 727-37 | lld:pubmed |
| pubmed-article:15349913 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:15349913 | pubmed:meshHeading | pubmed-meshheading:15349913... | lld:pubmed |
| pubmed-article:15349913 | pubmed:meshHeading | pubmed-meshheading:15349913... | lld:pubmed |
| pubmed-article:15349913 | pubmed:meshHeading | pubmed-meshheading:15349913... | lld:pubmed |
| pubmed-article:15349913 | pubmed:meshHeading | pubmed-meshheading:15349913... | lld:pubmed |
| pubmed-article:15349913 | pubmed:meshHeading | pubmed-meshheading:15349913... | lld:pubmed |
| pubmed-article:15349913 | pubmed:meshHeading | pubmed-meshheading:15349913... | lld:pubmed |
| pubmed-article:15349913 | pubmed:meshHeading | pubmed-meshheading:15349913... | lld:pubmed |
| pubmed-article:15349913 | pubmed:meshHeading | pubmed-meshheading:15349913... | lld:pubmed |
| pubmed-article:15349913 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15349913 | pubmed:articleTitle | Quantitation of covalently closed circular hepatitis B virus DNA in chronic hepatitis B patients. | lld:pubmed |
| pubmed-article:15349913 | pubmed:affiliation | Division of Gastroenterology and Hepatology, Department of Medicine, The University of Hong Kong, Queen Mary Hospital, Hong Kong, China. | lld:pubmed |
| pubmed-article:15349913 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15349913 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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