| pubmed-article:15341925 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15341925 | lifeskim:mentions | umls-concept:C0023418 | lld:lifeskim |
| pubmed-article:15341925 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:15341925 | lifeskim:mentions | umls-concept:C0162638 | lld:lifeskim |
| pubmed-article:15341925 | lifeskim:mentions | umls-concept:C0597225 | lld:lifeskim |
| pubmed-article:15341925 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:15341925 | lifeskim:mentions | umls-concept:C0070716 | lld:lifeskim |
| pubmed-article:15341925 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:15341925 | pubmed:dateCreated | 2004-9-2 | lld:pubmed |
| pubmed-article:15341925 | pubmed:abstractText | The mechanism of cell death by pheophorbide a (Pba) which has been established to be a potential photosensitizer was examined in experimental photodynamic therapy (PDT) on Jurkat cells, a human lymphoid tumor cell line. In 30-60 min after irradiation, Pba treated cells exhibited apoptotic features including membrane blebbing and DNA fragmentation. Pba/PDT caused a rapid release of cytochrome c from mitochondria into the cytosol. Sequentially, activation of caspase-3 and the cleavage of poly ADP-ribose polymerase (PARP) were followed. Meanwhile, no evidence of activation of caspase-8 was indicated in the cells. In experiments with caspase inhibitors, it was found that caspase-3 alone was sufficient initiator for the Pba-induced apoptosis of the cells. Pba specific emission spectra were confirmed in the mitochondrial fraction and the light irradiation caused a rapid change in its membrane potential. Thus, mitochondria were entailed as the crucial targets for Pba as well as a responsible component for the cytochrome c release to initiate apoptotic pathways. Taken together, it was concluded that the mode of Jurkat cell death by Pba/PDT is an apoptosis, which is initiated by mitochondrial cytochrome c release and caspase-3-pathways. | lld:pubmed |
| pubmed-article:15341925 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15341925 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15341925 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15341925 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15341925 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15341925 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15341925 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15341925 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15341925 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15341925 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15341925 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15341925 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:15341925 | pubmed:issn | 1011-1344 | lld:pubmed |
| pubmed-article:15341925 | pubmed:author | pubmed-author:LeeDai WoonDW | lld:pubmed |
| pubmed-article:15341925 | pubmed:author | pubmed-author:KimYong-RokYR | lld:pubmed |
| pubmed-article:15341925 | pubmed:author | pubmed-author:LimDae-SeogDS | lld:pubmed |
| pubmed-article:15341925 | pubmed:author | pubmed-author:KoSi-HwanSH | lld:pubmed |
| pubmed-article:15341925 | pubmed:author | pubmed-author:ParkYoung-Jae... | lld:pubmed |
| pubmed-article:15341925 | pubmed:author | pubmed-author:LeeWon-YoungW... | lld:pubmed |
| pubmed-article:15341925 | pubmed:author | pubmed-author:RyuKang-SunKS | lld:pubmed |
| pubmed-article:15341925 | pubmed:author | pubmed-author:AhnMi-YoungMY | lld:pubmed |
| pubmed-article:15341925 | pubmed:author | pubmed-author:ChoChang-Whan... | lld:pubmed |
| pubmed-article:15341925 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15341925 | pubmed:day | 8 | lld:pubmed |
| pubmed-article:15341925 | pubmed:volume | 75 | lld:pubmed |
| pubmed-article:15341925 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15341925 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15341925 | pubmed:pagination | 119-26 | lld:pubmed |
| pubmed-article:15341925 | pubmed:dateRevised | 2007-7-23 | lld:pubmed |
| pubmed-article:15341925 | pubmed:meshHeading | pubmed-meshheading:15341925... | lld:pubmed |
| pubmed-article:15341925 | pubmed:meshHeading | pubmed-meshheading:15341925... | lld:pubmed |
| pubmed-article:15341925 | pubmed:meshHeading | pubmed-meshheading:15341925... | lld:pubmed |
| pubmed-article:15341925 | pubmed:meshHeading | pubmed-meshheading:15341925... | lld:pubmed |
| pubmed-article:15341925 | pubmed:meshHeading | pubmed-meshheading:15341925... | lld:pubmed |
| pubmed-article:15341925 | pubmed:meshHeading | pubmed-meshheading:15341925... | lld:pubmed |
| pubmed-article:15341925 | pubmed:meshHeading | pubmed-meshheading:15341925... | lld:pubmed |
| pubmed-article:15341925 | pubmed:meshHeading | pubmed-meshheading:15341925... | lld:pubmed |
| pubmed-article:15341925 | pubmed:meshHeading | pubmed-meshheading:15341925... | lld:pubmed |
| pubmed-article:15341925 | pubmed:meshHeading | pubmed-meshheading:15341925... | lld:pubmed |
| pubmed-article:15341925 | pubmed:meshHeading | pubmed-meshheading:15341925... | lld:pubmed |
| pubmed-article:15341925 | pubmed:meshHeading | pubmed-meshheading:15341925... | lld:pubmed |
| pubmed-article:15341925 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15341925 | pubmed:articleTitle | Photoactivation of pheophorbide a induces a mitochondrial-mediated apoptosis in Jurkat leukaemia cells. | lld:pubmed |
| pubmed-article:15341925 | pubmed:affiliation | Department of Microbiology, College of Medicine, Yonsei University, CPO Box 8044, Seoul 120-752, Republic of Korea. | lld:pubmed |
| pubmed-article:15341925 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15341925 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:15341925 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15341925 | lld:pubmed |
