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pubmed-article:15332630pubmed:dateCreated2004-8-30lld:pubmed
pubmed-article:15332630pubmed:abstractTextWe illustrate some of the uses of micro-computed tomography (micro-CT) to study tissue-engineered bone using a micro-CT facility for imaging and visualizing biomaterials in three dimensions (3-D). The micro-CT is capable of acquiring 3D X-ray CT images made up of 2000(3) voxels on specimens up to 5 cm in extent with resolutions down to 2 microm. This allows the 3-D structure of tissue-engineered materials to be imaged across orders of magnitude in resolution. This capability is used to examine an explanted, tissue-engineered bone material based on a polycaprolactone scaffold and autologous bone marrow cells. Imaging of the tissue-engineered bone at a scale of 1 cm and resolutions of 10 microm allows one to visualize the complex ingrowth of bone into the polymer scaffold. From a theoretical viewpoint the voxel data may also be used to calculate expected mechanical properties of the tissue-engineered implant. These observations illustrate the benefits of tomography over traditional techniques for the characterization of bone morphology and interconnectivity. As the method is nondestructive it can perform a complimentary role to current histomorphometric techniques.lld:pubmed
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pubmed-article:15332630pubmed:pagination529-32lld:pubmed
pubmed-article:15332630pubmed:dateRevised2008-8-14lld:pubmed
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pubmed-article:15332630pubmed:year2004lld:pubmed
pubmed-article:15332630pubmed:articleTitleInvestigation of microstructural features in regenerating bone using micro computed tomography.lld:pubmed
pubmed-article:15332630pubmed:affiliationDepartment of Applied Mathematics, Research School of Physical Sciences and Engineering, Australian National University, ACT 0200, Australia.lld:pubmed
pubmed-article:15332630pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:15332630pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
pubmed-article:15332630pubmed:publicationTypeEvaluation Studieslld:pubmed
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