| pubmed-article:15331324 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15331324 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:15331324 | lifeskim:mentions | umls-concept:C0023820 | lld:lifeskim |
| pubmed-article:15331324 | lifeskim:mentions | umls-concept:C0205147 | lld:lifeskim |
| pubmed-article:15331324 | lifeskim:mentions | umls-concept:C1819439 | lld:lifeskim |
| pubmed-article:15331324 | lifeskim:mentions | umls-concept:C1305923 | lld:lifeskim |
| pubmed-article:15331324 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:15331324 | pubmed:dateCreated | 2004-8-27 | lld:pubmed |
| pubmed-article:15331324 | pubmed:abstractText | Mycobacterium leprae lipoprotein, LpK, induced IL-12 production from human monocytes. To determine the components essential for cytokine production and the relative role of lipidation in the activation process, we produced lipidated and non-lipidated truncated forms of LpK. While 0.5nM of lipidated LpK-a having N-terminal 60 amino acids of LpK produced more than 700pg/ml IL-12 p40, the non-lipidated LpK-b having the same amino acids as that of LpK-a required more than 20nM of the protein to produce an equivalent dose of cytokine. Truncated protein having the C-terminal 192 amino acids of LpK did not induce any cytokine production. Fifty nanomolar of the synthetic lipopeptide of LpK produced only about 200pg/ml IL-12. Among the truncated LpK, only LpK-a and lipopeptide stimulated NF-kB-dependent reporter activity in TLR-2 transfectant. However, when monocytes were stimulated with lipopeptide in the presence of non-lipidated protein, they produced IL-12 synergistically. Therefore, both peptide regions of LpK and lipid residues are necessary for efficient IL-12 production. | lld:pubmed |
| pubmed-article:15331324 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15331324 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15331324 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15331324 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15331324 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15331324 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15331324 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15331324 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15331324 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15331324 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15331324 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15331324 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15331324 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15331324 | pubmed:month | May | lld:pubmed |
| pubmed-article:15331324 | pubmed:issn | 0008-8749 | lld:pubmed |
| pubmed-article:15331324 | pubmed:author | pubmed-author:MakinoMasahik... | lld:pubmed |
| pubmed-article:15331324 | pubmed:author | pubmed-author:BrennanPatric... | lld:pubmed |
| pubmed-article:15331324 | pubmed:author | pubmed-author:TakeshitaFumi... | lld:pubmed |
| pubmed-article:15331324 | pubmed:author | pubmed-author:MaedaYumiY | lld:pubmed |
| pubmed-article:15331324 | pubmed:author | pubmed-author:YamashitaYasu... | lld:pubmed |
| pubmed-article:15331324 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15331324 | pubmed:volume | 229 | lld:pubmed |
| pubmed-article:15331324 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15331324 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15331324 | pubmed:pagination | 13-20 | lld:pubmed |
| pubmed-article:15331324 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:15331324 | pubmed:meshHeading | pubmed-meshheading:15331324... | lld:pubmed |
| pubmed-article:15331324 | pubmed:meshHeading | pubmed-meshheading:15331324... | lld:pubmed |
| pubmed-article:15331324 | pubmed:meshHeading | pubmed-meshheading:15331324... | lld:pubmed |
| pubmed-article:15331324 | pubmed:meshHeading | pubmed-meshheading:15331324... | lld:pubmed |
| pubmed-article:15331324 | pubmed:meshHeading | pubmed-meshheading:15331324... | lld:pubmed |
| pubmed-article:15331324 | pubmed:meshHeading | pubmed-meshheading:15331324... | lld:pubmed |
| pubmed-article:15331324 | pubmed:meshHeading | pubmed-meshheading:15331324... | lld:pubmed |
| pubmed-article:15331324 | pubmed:meshHeading | pubmed-meshheading:15331324... | lld:pubmed |
| pubmed-article:15331324 | pubmed:meshHeading | pubmed-meshheading:15331324... | lld:pubmed |
| pubmed-article:15331324 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15331324 | pubmed:articleTitle | Role of the polypeptide region of a 33kDa mycobacterial lipoprotein for efficient IL-12 production. | lld:pubmed |
| pubmed-article:15331324 | pubmed:affiliation | Department of Microbiology, Leprosy Research Center,National Institute of Infectious Diseases, 4-2-1 Aobacho,Higashimurayama, Tokyo 189-0002, Japan. | lld:pubmed |
| pubmed-article:15331324 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15331324 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15331324 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15331324 | lld:pubmed |
