| pubmed-article:15250493 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15250493 | lifeskim:mentions | umls-concept:C0086376 | lld:lifeskim |
| pubmed-article:15250493 | lifeskim:mentions | umls-concept:C0392747 | lld:lifeskim |
| pubmed-article:15250493 | lifeskim:mentions | umls-concept:C1511539 | lld:lifeskim |
| pubmed-article:15250493 | lifeskim:mentions | umls-concept:C0178463 | lld:lifeskim |
| pubmed-article:15250493 | lifeskim:mentions | umls-concept:C1554963 | lld:lifeskim |
| pubmed-article:15250493 | pubmed:dateCreated | 2004-7-14 | lld:pubmed |
| pubmed-article:15250493 | pubmed:abstractText | The activation of heterotrimeric G-proteins is tightly regulated by the exchange of GTP for GDP in the alpha-subunit; mostly--but not exclusively--seven-transmembrane receptors function as the guanine nucleotide exchange factors (GEFs). A research goal may be to determine which G-protein alpha-subunit is activated by the receptor under investigation. In a membrane preparation obtained from cells or tissues this can be achieved in a seemingly straightforward manner by determining if the receptor increases the covalent incorporation of GTP analogs into G-protein alpha-subunits. Because the GTP analogs may be labeled to high specific radioactivity the alpha-subunit can then be identified with the use of specific antibodies. One of the compounds we present here (2',3'-dialdehyde-GTP) can also be employed to block receptor-mediated G-protein activation and to disrupt the cognate signaling pathway. | lld:pubmed |
| pubmed-article:15250493 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15250493 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15250493 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15250493 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15250493 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15250493 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15250493 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15250493 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15250493 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15250493 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15250493 | pubmed:issn | 1064-3745 | lld:pubmed |
| pubmed-article:15250493 | pubmed:author | pubmed-author:NanoffChristi... | lld:pubmed |
| pubmed-article:15250493 | pubmed:author | pubmed-author:FreissmuthMic... | lld:pubmed |
| pubmed-article:15250493 | pubmed:author | pubmed-author:HoheneggerMar... | lld:pubmed |
| pubmed-article:15250493 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15250493 | pubmed:volume | 259 | lld:pubmed |
| pubmed-article:15250493 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15250493 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15250493 | pubmed:pagination | 183-95 | lld:pubmed |
| pubmed-article:15250493 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
| pubmed-article:15250493 | pubmed:meshHeading | pubmed-meshheading:15250493... | lld:pubmed |
| pubmed-article:15250493 | pubmed:meshHeading | pubmed-meshheading:15250493... | lld:pubmed |
| pubmed-article:15250493 | pubmed:meshHeading | pubmed-meshheading:15250493... | lld:pubmed |
| pubmed-article:15250493 | pubmed:meshHeading | pubmed-meshheading:15250493... | lld:pubmed |
| pubmed-article:15250493 | pubmed:meshHeading | pubmed-meshheading:15250493... | lld:pubmed |
| pubmed-article:15250493 | pubmed:meshHeading | pubmed-meshheading:15250493... | lld:pubmed |
| pubmed-article:15250493 | pubmed:meshHeading | pubmed-meshheading:15250493... | lld:pubmed |
| pubmed-article:15250493 | pubmed:meshHeading | pubmed-meshheading:15250493... | lld:pubmed |
| pubmed-article:15250493 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15250493 | pubmed:articleTitle | Covalent modification of G-proteins by affinity labeling. | lld:pubmed |
| pubmed-article:15250493 | pubmed:affiliation | Institute of Pharmacology, Vienna University, Austria. | lld:pubmed |
| pubmed-article:15250493 | pubmed:publicationType | Journal Article | lld:pubmed |
