| pubmed-article:15154845 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15154845 | lifeskim:mentions | umls-concept:C0020944 | lld:lifeskim |
| pubmed-article:15154845 | lifeskim:mentions | umls-concept:C0996819 | lld:lifeskim |
| pubmed-article:15154845 | lifeskim:mentions | umls-concept:C0011050 | lld:lifeskim |
| pubmed-article:15154845 | lifeskim:mentions | umls-concept:C0041942 | lld:lifeskim |
| pubmed-article:15154845 | lifeskim:mentions | umls-concept:C0041945 | lld:lifeskim |
| pubmed-article:15154845 | lifeskim:mentions | umls-concept:C0681916 | lld:lifeskim |
| pubmed-article:15154845 | lifeskim:mentions | umls-concept:C0750572 | lld:lifeskim |
| pubmed-article:15154845 | lifeskim:mentions | umls-concept:C0030351 | lld:lifeskim |
| pubmed-article:15154845 | lifeskim:mentions | umls-concept:C0680844 | lld:lifeskim |
| pubmed-article:15154845 | lifeskim:mentions | umls-concept:C1705490 | lld:lifeskim |
| pubmed-article:15154845 | pubmed:issue | Pt 3 | lld:pubmed |
| pubmed-article:15154845 | pubmed:dateCreated | 2004-5-24 | lld:pubmed |
| pubmed-article:15154845 | pubmed:abstractText | Pigeonpea ( Cajanus cajan ) urease was immobilized on 1 cmx1 cm DEAE-cellulose paper strips. The optimum immobilization (51% activity) was observed at 4 degrees C, with a protein concentration of 1.0 mg/strip. The apparent optimum pH shifted from 7.3 to 6.8. Immobilized urease showed an optimal stability temperature of 67 degrees C, compared with 47 degrees C for the soluble urease. Time-dependent kinetics of the thermal inactivation of the immobilized urease were examined and found to be monophasic as compared with the soluble enzyme, which was biphasic. The Michaelis constant ( K (m)) for the DEAE-cellulose-immobilized urease was found to be 4.75 mM, 1.5 times higher than the soluble enzyme. Immobilized strips stored at 4 degrees C showed an increased half-life ( t (1/2)=150 days). There was practically no leaching of the enzyme from the immobilized strips over a period of 2 weeks. These strips were used for estimating the urea content of blood samples; the results obtained matched well with those obtained in a clinical laboratory through an Autoanalyzer(R) (Zydus Co., Rome, Italy). The easy availability of pigeonpea urease, the ease of its immobilization on DEAE-cellulose strips and the significantly lower cost of urease described in the present study makes it a suitable product for future applications in diagnostics. | lld:pubmed |
| pubmed-article:15154845 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15154845 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15154845 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15154845 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15154845 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15154845 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15154845 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15154845 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15154845 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:15154845 | pubmed:issn | 0885-4513 | lld:pubmed |
| pubmed-article:15154845 | pubmed:author | pubmed-author:SrivastavaPun... | lld:pubmed |
| pubmed-article:15154845 | pubmed:author | pubmed-author:KayasthaArvin... | lld:pubmed |
| pubmed-article:15154845 | pubmed:author | pubmed-author:Reddy KRavi... | lld:pubmed |
| pubmed-article:15154845 | pubmed:author | pubmed-author:DeyPrakash... | lld:pubmed |
| pubmed-article:15154845 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15154845 | pubmed:volume | 39 | lld:pubmed |
| pubmed-article:15154845 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15154845 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15154845 | pubmed:pagination | 323-7 | lld:pubmed |
| pubmed-article:15154845 | pubmed:dateRevised | 2007-3-21 | lld:pubmed |
| pubmed-article:15154845 | pubmed:meshHeading | pubmed-meshheading:15154845... | lld:pubmed |
| pubmed-article:15154845 | pubmed:meshHeading | pubmed-meshheading:15154845... | lld:pubmed |
| pubmed-article:15154845 | pubmed:meshHeading | pubmed-meshheading:15154845... | lld:pubmed |
| pubmed-article:15154845 | pubmed:meshHeading | pubmed-meshheading:15154845... | lld:pubmed |
| pubmed-article:15154845 | pubmed:meshHeading | pubmed-meshheading:15154845... | lld:pubmed |
| pubmed-article:15154845 | pubmed:meshHeading | pubmed-meshheading:15154845... | lld:pubmed |
| pubmed-article:15154845 | pubmed:meshHeading | pubmed-meshheading:15154845... | lld:pubmed |
| pubmed-article:15154845 | pubmed:meshHeading | pubmed-meshheading:15154845... | lld:pubmed |
| pubmed-article:15154845 | pubmed:meshHeading | pubmed-meshheading:15154845... | lld:pubmed |
| pubmed-article:15154845 | pubmed:meshHeading | pubmed-meshheading:15154845... | lld:pubmed |
| pubmed-article:15154845 | pubmed:meshHeading | pubmed-meshheading:15154845... | lld:pubmed |
| pubmed-article:15154845 | pubmed:meshHeading | pubmed-meshheading:15154845... | lld:pubmed |
| pubmed-article:15154845 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15154845 | pubmed:articleTitle | Immobilization of pigeonpea (Cajanus cajan) urease on DEAE-cellulose paper strips for urea estimation. | lld:pubmed |
| pubmed-article:15154845 | pubmed:affiliation | School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi-221 005, India. | lld:pubmed |
| pubmed-article:15154845 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15154845 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15154845 | lld:pubmed |
