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pubmed-article:15080836pubmed:abstractTextBlo t 11 is a high molecular weight allergen from Blomia tropicalis with significant immunoglobulin (Ig)E binding frequency. Native and recombinant Blo t 11 are susceptible to degradation and the isolation and expression of the allergen is problematic thus obtaining sufficient amounts of purified Blo t 11 for antibody production is limiting. DNA-based immunization is an attractive alternative strategy that bypasses antigen purification for antibody production.lld:pubmed
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pubmed-article:15080836pubmed:articleTitleDNA immunization for the production of monoclonal antibodies to Blo t 11, a paramyosin homolog from Blomia tropicalis.lld:pubmed
pubmed-article:15080836pubmed:affiliationDepartment of Paediatrics, National University of Singapore, Singapore.lld:pubmed
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