| pubmed-article:15070671 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15070671 | lifeskim:mentions | umls-concept:C1113687 | lld:lifeskim |
| pubmed-article:15070671 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:15070671 | lifeskim:mentions | umls-concept:C1704864 | lld:lifeskim |
| pubmed-article:15070671 | lifeskim:mentions | umls-concept:C1710548 | lld:lifeskim |
| pubmed-article:15070671 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:15070671 | pubmed:dateCreated | 2004-4-8 | lld:pubmed |
| pubmed-article:15070671 | pubmed:abstractText | Human embryonic stem (huES) cells have the ability to differentiate into a variety of cell lineages and potentially provide a source of differentiated cells for many therapeutic uses. However, little is known about the mechanism of differentiation of huES cells and factors regulating cell development. We have used high-quality microarrays containing 16 659 seventy-base pair oligonucleotides to examine gene expression in 6 of the 11 available huES cell lines. Expression was compared against pooled RNA from multiple tissues (universal RNA) and genes enriched in huES cells were identified. All 6 cell lines expressed multiple markers of the undifferentiated state and shared significant homology in gene expression (overall similarity coefficient > 0.85).A common subset of 92 genes was identified that included Nanog, GTCM-1, connexin 43 (GJA1), oct-4, and TDGF1 (cripto). Gene expression was confirmed by a variety of techniques including comparison with databases, reverse transcriptase-polymerase chain reaction, focused cDNA microarrays, and immunocytochemistry. Comparison with published "stemness" genes revealed a limited overlap, suggesting little similarity with other stem cell populations. Several novel ES cell-specific expressed sequence tags were identified and mapped to the human genome. These results represent the first detailed characterization of undifferentiated huES cells and provide a unique set of markers to profile and better understand the biology of huES cells. | lld:pubmed |
| pubmed-article:15070671 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15070671 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15070671 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15070671 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:15070671 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15070671 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15070671 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:15070671 | pubmed:issn | 0006-4971 | lld:pubmed |
| pubmed-article:15070671 | pubmed:author | pubmed-author:ThiesR... | lld:pubmed |
| pubmed-article:15070671 | pubmed:author | pubmed-author:PuriRaj KRK | lld:pubmed |
| pubmed-article:15070671 | pubmed:author | pubmed-author:GinisIreneI | lld:pubmed |
| pubmed-article:15070671 | pubmed:author | pubmed-author:JoshiBharat... | lld:pubmed |
| pubmed-article:15070671 | pubmed:author | pubmed-author:RaoMahendra... | lld:pubmed |
| pubmed-article:15070671 | pubmed:author | pubmed-author:LuoYongquanY | lld:pubmed |
| pubmed-article:15070671 | pubmed:author | pubmed-author:AmitMichalM | lld:pubmed |
| pubmed-article:15070671 | pubmed:author | pubmed-author:Itskovitz-Eld... | lld:pubmed |
| pubmed-article:15070671 | pubmed:author | pubmed-author:LyonsIanI | lld:pubmed |
| pubmed-article:15070671 | pubmed:author | pubmed-author:CondieBrian... | lld:pubmed |
| pubmed-article:15070671 | pubmed:author | pubmed-author:MiuraTakumiT | lld:pubmed |
| pubmed-article:15070671 | pubmed:author | pubmed-author:BhattacharyaB... | lld:pubmed |
| pubmed-article:15070671 | pubmed:author | pubmed-author:Brandenberger... | lld:pubmed |
| pubmed-article:15070671 | pubmed:author | pubmed-author:MejidoJosefJ | lld:pubmed |
| pubmed-article:15070671 | pubmed:author | pubmed-author:YangAmy XAX | lld:pubmed |
| pubmed-article:15070671 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15070671 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:15070671 | pubmed:volume | 103 | lld:pubmed |
| pubmed-article:15070671 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15070671 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15070671 | pubmed:pagination | 2956-64 | lld:pubmed |
| pubmed-article:15070671 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:15070671 | pubmed:meshHeading | pubmed-meshheading:15070671... | lld:pubmed |
| pubmed-article:15070671 | pubmed:meshHeading | pubmed-meshheading:15070671... | lld:pubmed |
| pubmed-article:15070671 | pubmed:meshHeading | pubmed-meshheading:15070671... | lld:pubmed |
| pubmed-article:15070671 | pubmed:meshHeading | pubmed-meshheading:15070671... | lld:pubmed |
| pubmed-article:15070671 | pubmed:meshHeading | pubmed-meshheading:15070671... | lld:pubmed |
| pubmed-article:15070671 | pubmed:meshHeading | pubmed-meshheading:15070671... | lld:pubmed |
| pubmed-article:15070671 | pubmed:meshHeading | pubmed-meshheading:15070671... | lld:pubmed |
| pubmed-article:15070671 | pubmed:meshHeading | pubmed-meshheading:15070671... | lld:pubmed |
| pubmed-article:15070671 | pubmed:meshHeading | pubmed-meshheading:15070671... | lld:pubmed |
| pubmed-article:15070671 | pubmed:meshHeading | pubmed-meshheading:15070671... | lld:pubmed |
| pubmed-article:15070671 | pubmed:meshHeading | pubmed-meshheading:15070671... | lld:pubmed |
| pubmed-article:15070671 | pubmed:meshHeading | pubmed-meshheading:15070671... | lld:pubmed |
| pubmed-article:15070671 | pubmed:meshHeading | pubmed-meshheading:15070671... | lld:pubmed |
| pubmed-article:15070671 | pubmed:meshHeading | pubmed-meshheading:15070671... | lld:pubmed |
| pubmed-article:15070671 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15070671 | pubmed:articleTitle | Gene expression in human embryonic stem cell lines: unique molecular signature. | lld:pubmed |
| pubmed-article:15070671 | pubmed:affiliation | Laboratory of Molecular Tumor Biology, Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA. | lld:pubmed |
| pubmed-article:15070671 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15070671 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:15070671 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:15070671 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15070671 | lld:pubmed |
