| pubmed-article:14975753 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14975753 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:14975753 | lifeskim:mentions | umls-concept:C0018957 | lld:lifeskim |
| pubmed-article:14975753 | lifeskim:mentions | umls-concept:C0042971 | lld:lifeskim |
| pubmed-article:14975753 | lifeskim:mentions | umls-concept:C0993609 | lld:lifeskim |
| pubmed-article:14975753 | lifeskim:mentions | umls-concept:C1947906 | lld:lifeskim |
| pubmed-article:14975753 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:14975753 | pubmed:dateCreated | 2004-2-20 | lld:pubmed |
| pubmed-article:14975753 | pubmed:abstractText | The aim of this work was to investigate sorting mechanisms of von Willebrand factor (VWF) when expressed in haematopoietic cells. The processing and sorting of both the wild-type VWF and a multimerization defective propeptide-mutant (VWF(m)) were investigated after expression in the 32D cell line. Normal proteolytic processing was observed for both proteins, however the processing of VWF(m) was much slower and a large portion was unprocessed. Results from subcellular fractionation and immunoelectron microscopy confirmed that a part of VWF, but not VWF(m), was targeted to lysosome-related granules. Partial constitutive secretion was also observed for all forms of VWF and VWF(m). Inhibition of acidification by chloroquine blocked VWF processing but allowed unprocessed pro-VWF targeting to dense organelles. In conclusion, our observations are consistent with VWF multimerization being of importance in cellular retention and targeting to lysosome-related organelles in haematopoietic cells, suggesting a role of protein aggregation for sorting in these cells. | lld:pubmed |
| pubmed-article:14975753 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14975753 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14975753 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:14975753 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14975753 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14975753 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14975753 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14975753 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14975753 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14975753 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:14975753 | pubmed:issn | 0006-291X | lld:pubmed |
| pubmed-article:14975753 | pubmed:author | pubmed-author:HolmbergLarsL | lld:pubmed |
| pubmed-article:14975753 | pubmed:author | pubmed-author:CalafatJeroJ | lld:pubmed |
| pubmed-article:14975753 | pubmed:author | pubmed-author:OlssonIngeI | lld:pubmed |
| pubmed-article:14975753 | pubmed:author | pubmed-author:RosénHannaH | lld:pubmed |
| pubmed-article:14975753 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14975753 | pubmed:day | 12 | lld:pubmed |
| pubmed-article:14975753 | pubmed:volume | 315 | lld:pubmed |
| pubmed-article:14975753 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14975753 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14975753 | pubmed:pagination | 671-8 | lld:pubmed |
| pubmed-article:14975753 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:14975753 | pubmed:meshHeading | pubmed-meshheading:14975753... | lld:pubmed |
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| pubmed-article:14975753 | pubmed:meshHeading | pubmed-meshheading:14975753... | lld:pubmed |
| pubmed-article:14975753 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:14975753 | pubmed:articleTitle | Sorting of Von Willebrand factor to lysosome-related granules of haematopoietic cells. | lld:pubmed |
| pubmed-article:14975753 | pubmed:affiliation | Department of Hematology, C14, BMC, S-221 84 Lund, Sweden. | lld:pubmed |
| pubmed-article:14975753 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14975753 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
