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pubmed-article:1492519pubmed:abstractTextA simple and efficient variant of the E-rosette test based on addition of dextran to the incubation media is described. This variant 1) does not include B cells 2) involves some CD2+ null cells as described 3) is not inhibited by anti-CD5 antibody 4) correlates with CD2 expression 5) detects specific changes in the relative proportion of T-lymphocytes under the different conditions. In a group of calves the mean percentage of RFC in the peripheral blood lymphocytes was 53.59 +/- 8.70 and in cows there was 72.57 +/- 3.85. The proportion of RFC detected in bovine leukemia virus (BLV)--infected cows with lymphocytosis was less than one third of that in BLV--negative animals and vice versa in B (MHC class II+) lymphocytes.lld:pubmed
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pubmed-article:1492519pubmed:monthNovlld:pubmed
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pubmed-article:1492519pubmed:volume39lld:pubmed
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pubmed-article:1492519pubmed:pagination633-40lld:pubmed
pubmed-article:1492519pubmed:dateRevised2008-2-13lld:pubmed
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pubmed-article:1492519pubmed:year1992lld:pubmed
pubmed-article:1492519pubmed:articleTitleDetection of T-lymphocytes in the peripheral blood and lymphoid tissues of cattle using a variant of the E-rosette test and monoclonal antibodies.lld:pubmed
pubmed-article:1492519pubmed:affiliationUniversity of Veterinary Science, Institute of Animal Breeding and Genetics, Brno, CSFR.lld:pubmed
pubmed-article:1492519pubmed:publicationTypeJournal Articlelld:pubmed