pubmed-article:14676403 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:14676403 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
pubmed-article:14676403 | lifeskim:mentions | umls-concept:C1522449 | lld:lifeskim |
pubmed-article:14676403 | lifeskim:mentions | umls-concept:C0237753 | lld:lifeskim |
pubmed-article:14676403 | lifeskim:mentions | umls-concept:C0600627 | lld:lifeskim |
pubmed-article:14676403 | pubmed:issue | 3 | lld:pubmed |
pubmed-article:14676403 | pubmed:dateCreated | 2003-12-16 | lld:pubmed |
pubmed-article:14676403 | pubmed:abstractText | A single cell irradiation system has been developed at JAERI-Takasaki to elucidate biological effects of low dose and low dose-rate space radiation. The system has been installed at high-energy collimated heavy-ion microbeam apparatus under a vertical beam line of AVF-cyclotron accelerator. Individual mammalian cultured cells are irradiated in the atmosphere on the cell dish, the bottom of which is made of ion-track-detector TNF-1 (modified CR-39), with a single or precise numbers of 11.5 MeV/u 40Ar13+, 13.0 MeV/u 20Ne7+, and 18.3 MeV/u 12C5+ ions. Positional data of the target cells are obtained at the off-line microscope before irradiation. Targeting and irradiation of the cells are performed automatically at the on-line microscope of the microbeam apparatus according to the obtained data. About 50-100 cells are irradiated in several minutes. The number of ions traversed the cells attached on the ion track detector were counted with a plastic scintillator-photomultiplier tube assembly and a constant fraction discriminator. Immediately after the irradiation, the bottom of the cell dish was etched at 37 degrees C to check the spatial distribution of irradiated ions. The one batch of cell sample is treated in about 1 hr including the phases of cell finding, irradiation, and etching/observing of the ion tracks. | lld:pubmed |
pubmed-article:14676403 | pubmed:language | eng | lld:pubmed |
pubmed-article:14676403 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:14676403 | pubmed:citationSubset | S | lld:pubmed |
pubmed-article:14676403 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:14676403 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:14676403 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:14676403 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:14676403 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:14676403 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:14676403 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:14676403 | pubmed:month | Oct | lld:pubmed |
pubmed-article:14676403 | pubmed:issn | 0914-9201 | lld:pubmed |
pubmed-article:14676403 | pubmed:author | pubmed-author:KobayashiYasu... | lld:pubmed |
pubmed-article:14676403 | pubmed:author | pubmed-author:FunayamaTomoo... | lld:pubmed |
pubmed-article:14676403 | pubmed:author | pubmed-author:WadaSeiichiS | lld:pubmed |
pubmed-article:14676403 | pubmed:author | pubmed-author:SakashitaTets... | lld:pubmed |
pubmed-article:14676403 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:14676403 | pubmed:volume | 17 | lld:pubmed |
pubmed-article:14676403 | pubmed:owner | NASA | lld:pubmed |
pubmed-article:14676403 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:14676403 | pubmed:pagination | 253-4 | lld:pubmed |
pubmed-article:14676403 | pubmed:dateRevised | 2009-6-4 | lld:pubmed |
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pubmed-article:14676403 | pubmed:year | 2003 | lld:pubmed |
pubmed-article:14676403 | pubmed:articleTitle | System of cell irradiation with a precise number of heavy ions (II). | lld:pubmed |
pubmed-article:14676403 | pubmed:affiliation | Research Group for Biotechnology Development, Japan Atomic Energy Research Institute (JAERI-Takasaki). | lld:pubmed |
pubmed-article:14676403 | pubmed:publicationType | Journal Article | lld:pubmed |