| pubmed-article:14675756 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14675756 | lifeskim:mentions | umls-concept:C0162741 | lld:lifeskim |
| pubmed-article:14675756 | lifeskim:mentions | umls-concept:C0025723 | lld:lifeskim |
| pubmed-article:14675756 | lifeskim:mentions | umls-concept:C0014290 | lld:lifeskim |
| pubmed-article:14675756 | lifeskim:mentions | umls-concept:C0376249 | lld:lifeskim |
| pubmed-article:14675756 | lifeskim:mentions | umls-concept:C0205251 | lld:lifeskim |
| pubmed-article:14675756 | lifeskim:mentions | umls-concept:C1707520 | lld:lifeskim |
| pubmed-article:14675756 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:14675756 | lifeskim:mentions | umls-concept:C1705938 | lld:lifeskim |
| pubmed-article:14675756 | lifeskim:mentions | umls-concept:C1527178 | lld:lifeskim |
| pubmed-article:14675756 | lifeskim:mentions | umls-concept:C0075739 | lld:lifeskim |
| pubmed-article:14675756 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:14675756 | pubmed:dateCreated | 2003-12-16 | lld:pubmed |
| pubmed-article:14675756 | pubmed:abstractText | A powerful system to create gain-of-function mutants in plants is activation tagging using T-DNA based vehicles to introduce transcriptional enhancer sequences. Large Arabidopsis populations of individual plants carrying a quadruple cauliflower mosaic virus (CaMV) 35S enhancer are frequently used for mutant screenings, however the frequency of morphological mutants remains very low. To clarify this low frequency we analyzed a subset of lines generated by this method. The correlation between the number of T-DNA insertion sites, the methylation status of the 35S enhancer sequence and 35S enhancer activity was determined. All plants containing more than a single T-DNA insertion showed methylation of the 35S enhancer and revealed a dramatic decrease in 35S enhancer activity. The results support the notion that in a large proportion of the T-DNA based activation tagged lines the 35S transcriptional enhancer is silenced due to methylation, which is induced by multiple T-DNA integrations. | lld:pubmed |
| pubmed-article:14675756 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14675756 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14675756 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:14675756 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14675756 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14675756 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14675756 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14675756 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:14675756 | pubmed:issn | 0014-5793 | lld:pubmed |
| pubmed-article:14675756 | pubmed:author | pubmed-author:MlynárováLudm... | lld:pubmed |
| pubmed-article:14675756 | pubmed:author | pubmed-author:AngenentGerco... | lld:pubmed |
| pubmed-article:14675756 | pubmed:author | pubmed-author:AartsMark G... | lld:pubmed |
| pubmed-article:14675756 | pubmed:author | pubmed-author:Chalfun-Junio... | lld:pubmed |
| pubmed-article:14675756 | pubmed:author | pubmed-author:MesJurriaan... | lld:pubmed |
| pubmed-article:14675756 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14675756 | pubmed:day | 18 | lld:pubmed |
| pubmed-article:14675756 | pubmed:volume | 555 | lld:pubmed |
| pubmed-article:14675756 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14675756 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14675756 | pubmed:pagination | 459-63 | lld:pubmed |
| pubmed-article:14675756 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:14675756 | pubmed:meshHeading | pubmed-meshheading:14675756... | lld:pubmed |
| pubmed-article:14675756 | pubmed:meshHeading | pubmed-meshheading:14675756... | lld:pubmed |
| pubmed-article:14675756 | pubmed:meshHeading | pubmed-meshheading:14675756... | lld:pubmed |
| pubmed-article:14675756 | pubmed:meshHeading | pubmed-meshheading:14675756... | lld:pubmed |
| pubmed-article:14675756 | pubmed:meshHeading | pubmed-meshheading:14675756... | lld:pubmed |
| pubmed-article:14675756 | pubmed:meshHeading | pubmed-meshheading:14675756... | lld:pubmed |
| pubmed-article:14675756 | pubmed:meshHeading | pubmed-meshheading:14675756... | lld:pubmed |
| pubmed-article:14675756 | pubmed:meshHeading | pubmed-meshheading:14675756... | lld:pubmed |
| pubmed-article:14675756 | pubmed:meshHeading | pubmed-meshheading:14675756... | lld:pubmed |
| pubmed-article:14675756 | pubmed:meshHeading | pubmed-meshheading:14675756... | lld:pubmed |
| pubmed-article:14675756 | pubmed:meshHeading | pubmed-meshheading:14675756... | lld:pubmed |
| pubmed-article:14675756 | pubmed:meshHeading | pubmed-meshheading:14675756... | lld:pubmed |
| pubmed-article:14675756 | pubmed:meshHeading | pubmed-meshheading:14675756... | lld:pubmed |
| pubmed-article:14675756 | pubmed:meshHeading | pubmed-meshheading:14675756... | lld:pubmed |
| pubmed-article:14675756 | pubmed:meshHeading | pubmed-meshheading:14675756... | lld:pubmed |
| pubmed-article:14675756 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:14675756 | pubmed:articleTitle | Low frequency of T-DNA based activation tagging in Arabidopsis is correlated with methylation of CaMV 35S enhancer sequences. | lld:pubmed |
| pubmed-article:14675756 | pubmed:affiliation | Business Unit Bioscience, Plant Research International, Bornsesteeg 65, 6708 PD, Wageningen, The Netherlands. gerco.angenent@wur.nl | lld:pubmed |
| pubmed-article:14675756 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14675756 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14675756 | lld:pubmed |
