| pubmed-article:14673779 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14673779 | lifeskim:mentions | umls-concept:C0020944 | lld:lifeskim |
| pubmed-article:14673779 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:14673779 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:14673779 | lifeskim:mentions | umls-concept:C0872079 | lld:lifeskim |
| pubmed-article:14673779 | lifeskim:mentions | umls-concept:C1521827 | lld:lifeskim |
| pubmed-article:14673779 | lifeskim:mentions | umls-concept:C0185125 | lld:lifeskim |
| pubmed-article:14673779 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:14673779 | lifeskim:mentions | umls-concept:C1136348 | lld:lifeskim |
| pubmed-article:14673779 | lifeskim:mentions | umls-concept:C0205250 | lld:lifeskim |
| pubmed-article:14673779 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:14673779 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:14673779 | lifeskim:mentions | umls-concept:C0332324 | lld:lifeskim |
| pubmed-article:14673779 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:14673779 | pubmed:dateCreated | 2003-12-15 | lld:pubmed |
| pubmed-article:14673779 | pubmed:abstractText | We have developed a highly sensitive microarray protein chip, ProteoChip, coated with ProLinker, novel calixcrown derivatives with a bifunctional coupling property that permits efficient immobilization of capture proteins on solid matrixes and makes high-throughput analysis of protein-protein interactions possible. The analysis of quartz crystal microbalance showed that both monoclonal antibody (mAb) and antigen (Ag) bound to the gold film of the sensor surface coated with ProLinker B and that it is useful for studies of Ab-Ag interactions. ProteoChip, aminated glass slide coated with ProLinker A, was also demonstrated to be useful for preparation of high-density array spots by using a microarrayer and for analysis of analyte Ags either by direct or sandwich methods of fluorescence immunoassay. The detection sensitivity of ProteoChip was as low as 1-10 femtogram/mL of analyte protein, useful for detection of tumor markers. ProteoChip was also useful for studies of direct protein-protein interactions as demonstrated by analysis of integrin-extracellular matrix protein interaction. These experimental results suggest that ProteoChip is a powerful tool for development of chip-based lead screening microarrays to monitor protein-protein interactions (i.e. drug target) as well as for biomarker assays which require high detection sensitivity. | lld:pubmed |
| pubmed-article:14673779 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14673779 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14673779 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:14673779 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14673779 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14673779 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14673779 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14673779 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14673779 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:14673779 | pubmed:issn | 1615-9853 | lld:pubmed |
| pubmed-article:14673779 | pubmed:author | pubmed-author:KangIn-CheolI... | lld:pubmed |
| pubmed-article:14673779 | pubmed:author | pubmed-author:LeeEun... | lld:pubmed |
| pubmed-article:14673779 | pubmed:author | pubmed-author:LeeYoonsukY | lld:pubmed |
| pubmed-article:14673779 | pubmed:author | pubmed-author:ChoYong WanYW | lld:pubmed |
| pubmed-article:14673779 | pubmed:author | pubmed-author:MatsuiTakuyaT | lld:pubmed |
| pubmed-article:14673779 | pubmed:author | pubmed-author:KimTai-SunTS | lld:pubmed |
| pubmed-article:14673779 | pubmed:author | pubmed-author:HanMoon HiMH | lld:pubmed |
| pubmed-article:14673779 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14673779 | pubmed:volume | 3 | lld:pubmed |
| pubmed-article:14673779 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14673779 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14673779 | pubmed:pagination | 2289-304 | lld:pubmed |
| pubmed-article:14673779 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:14673779 | pubmed:meshHeading | pubmed-meshheading:14673779... | lld:pubmed |
| pubmed-article:14673779 | pubmed:meshHeading | pubmed-meshheading:14673779... | lld:pubmed |
| pubmed-article:14673779 | pubmed:meshHeading | pubmed-meshheading:14673779... | lld:pubmed |
| pubmed-article:14673779 | pubmed:meshHeading | pubmed-meshheading:14673779... | lld:pubmed |
| pubmed-article:14673779 | pubmed:meshHeading | pubmed-meshheading:14673779... | lld:pubmed |
| pubmed-article:14673779 | pubmed:meshHeading | pubmed-meshheading:14673779... | lld:pubmed |
| pubmed-article:14673779 | pubmed:meshHeading | pubmed-meshheading:14673779... | lld:pubmed |
| pubmed-article:14673779 | pubmed:meshHeading | pubmed-meshheading:14673779... | lld:pubmed |
| pubmed-article:14673779 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:14673779 | pubmed:articleTitle | ProteoChip: a highly sensitive protein microarray prepared by a novel method of protein immobilization for application of protein-protein interaction studies. | lld:pubmed |
| pubmed-article:14673779 | pubmed:affiliation | Proteogen, RM 1516 Windstone, Yangjae-dong, Seocho-ku, Seoul, Korea. | lld:pubmed |
| pubmed-article:14673779 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14673779 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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