| pubmed-article:14518759 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14518759 | lifeskim:mentions | umls-concept:C0087111 | lld:lifeskim |
| pubmed-article:14518759 | lifeskim:mentions | umls-concept:C0037098 | lld:lifeskim |
| pubmed-article:14518759 | lifeskim:mentions | umls-concept:C1704675 | lld:lifeskim |
| pubmed-article:14518759 | lifeskim:mentions | umls-concept:C1609982 | lld:lifeskim |
| pubmed-article:14518759 | lifeskim:mentions | umls-concept:C0392756 | lld:lifeskim |
| pubmed-article:14518759 | lifeskim:mentions | umls-concept:C2717789 | lld:lifeskim |
| pubmed-article:14518759 | lifeskim:mentions | umls-concept:C0218462 | lld:lifeskim |
| pubmed-article:14518759 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:14518759 | pubmed:dateCreated | 2003-10-1 | lld:pubmed |
| pubmed-article:14518759 | pubmed:abstractText | This study describes the thermal pretreatment of a silica gel between 150 and 800 degrees C before derivatization with dimethyloctadecylchlorosilane as a means of reducing residual silanol activity in HPLC bonded stationary phases. A time study was done from 12 to 48 h to find the optimum time needed for dehydroxylation. With increasing pretreatment temperatures, the number of reactive silanols is reduced from 8 micromol/m2 to essentially zero at 1000 degrees C (where sintering occurs). The effects of the thermal pretreatments were observed with diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) and solid state cross polarization magic angle spinning (CP-MAS) 29Si NMR. Following derivatization, residual silanol activity and pH stability were tested by packing columns with the derivatized silica and carrying out a series of reversed-phase liquid chromatography (RPLC) experiments. Residual silanol activity was greatly reduced when the silica was pretreated at 800 degrees C, leading to less peak tailing for basic solutes. In a strongly basic mobile phase (pH 11.5) the pretreated silica was surprisingly stable, although bond cleavage of C18 groups from the surface was observed. | lld:pubmed |
| pubmed-article:14518759 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14518759 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14518759 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:14518759 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14518759 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14518759 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14518759 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14518759 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:14518759 | pubmed:issn | 0021-9673 | lld:pubmed |
| pubmed-article:14518759 | pubmed:author | pubmed-author:CooperWilliam... | lld:pubmed |
| pubmed-article:14518759 | pubmed:author | pubmed-author:DorseyJohn... | lld:pubmed |
| pubmed-article:14518759 | pubmed:author | pubmed-author:SunseriJ... | lld:pubmed |
| pubmed-article:14518759 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14518759 | pubmed:day | 5 | lld:pubmed |
| pubmed-article:14518759 | pubmed:volume | 1011 | lld:pubmed |
| pubmed-article:14518759 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14518759 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14518759 | pubmed:pagination | 23-9 | lld:pubmed |
| pubmed-article:14518759 | pubmed:dateRevised | 2009-1-15 | lld:pubmed |
| pubmed-article:14518759 | pubmed:meshHeading | pubmed-meshheading:14518759... | lld:pubmed |
| pubmed-article:14518759 | pubmed:meshHeading | pubmed-meshheading:14518759... | lld:pubmed |
| pubmed-article:14518759 | pubmed:meshHeading | pubmed-meshheading:14518759... | lld:pubmed |
| pubmed-article:14518759 | pubmed:meshHeading | pubmed-meshheading:14518759... | lld:pubmed |
| pubmed-article:14518759 | pubmed:meshHeading | pubmed-meshheading:14518759... | lld:pubmed |
| pubmed-article:14518759 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:14518759 | pubmed:articleTitle | Reducing residual silanol interactions in reversed-phase liquid chromatography. Thermal treatment of silica before derivatization. | lld:pubmed |
| pubmed-article:14518759 | pubmed:affiliation | The Florida State University, Department of Chemistry and Biochemistry, Tallahassee, FL 32306-4390, USA. | lld:pubmed |
| pubmed-article:14518759 | pubmed:publicationType | Journal Article | lld:pubmed |
