Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:1444393rdf:typepubmed:Citationlld:pubmed
pubmed-article:1444393lifeskim:mentionsumls-concept:C0007634lld:lifeskim
pubmed-article:1444393lifeskim:mentionsumls-concept:C0016452lld:lifeskim
pubmed-article:1444393lifeskim:mentionsumls-concept:C0023861lld:lifeskim
pubmed-article:1444393lifeskim:mentionsumls-concept:C0025663lld:lifeskim
pubmed-article:1444393lifeskim:mentionsumls-concept:C1552130lld:lifeskim
pubmed-article:1444393lifeskim:mentionsumls-concept:C0032520lld:lifeskim
pubmed-article:1444393lifeskim:mentionsumls-concept:C1524062lld:lifeskim
pubmed-article:1444393lifeskim:mentionsumls-concept:C0182400lld:lifeskim
pubmed-article:1444393pubmed:issue9lld:pubmed
pubmed-article:1444393pubmed:dateCreated1992-12-23lld:pubmed
pubmed-article:1444393pubmed:abstractTextA rapid polymerase chain reaction (PCR) method was developed for detection of Listeria monocytogenes in foods. This method used a pair of primers based on a unique region in the 16S rRNA sequence of L. monocytogenes, which were previously reported by us to yield a specific nucleic acid probe. Our method included use of a shorter denaturing time, a shorter annealing time, a rapid transition, and an increase in the number of cycles, resulting in good sensitivity. Just 3 h for PCR plus 1 h for electrophoresis was required. Additional time for DNA isolation and DNA hybridization was not needed. This method detected as few as 2 to 20 CFU of L. monocytogenes in pure cultures and as few as 4 to 40 CFU of L. monocytogenes in inoculated (10(8) CFU), diluted food samples. Seven of eight foods, including four poultry products, gave positive results. Only one food sample, soft cheese, gave interference. An internal probe hybridization test was used to confirm that the PCR products were from L. monocytogenes. A specificity test indicated that this PCR method was positive for all 13 strains of L. monocytogenes tested but negative for the other 6 species of Listeria, including 6 strains of L. innocua, and negative for 17 other gram-positive and gram-negative bacteria tested.lld:pubmed
pubmed-article:1444393pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1444393pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1444393pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1444393pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1444393pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1444393pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1444393pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1444393pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1444393pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1444393pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1444393pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1444393pubmed:languageenglld:pubmed
pubmed-article:1444393pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1444393pubmed:citationSubsetIMlld:pubmed
pubmed-article:1444393pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1444393pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1444393pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1444393pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1444393pubmed:statusMEDLINElld:pubmed
pubmed-article:1444393pubmed:monthSeplld:pubmed
pubmed-article:1444393pubmed:issn0099-2240lld:pubmed
pubmed-article:1444393pubmed:authorpubmed-author:JohnsonM GMGlld:pubmed
pubmed-article:1444393pubmed:authorpubmed-author:WangR FRFlld:pubmed
pubmed-article:1444393pubmed:authorpubmed-author:XinJ XJXlld:pubmed
pubmed-article:1444393pubmed:issnTypePrintlld:pubmed
pubmed-article:1444393pubmed:volume58lld:pubmed
pubmed-article:1444393pubmed:ownerNLMlld:pubmed
pubmed-article:1444393pubmed:authorsCompleteYlld:pubmed
pubmed-article:1444393pubmed:pagination2827-31lld:pubmed
pubmed-article:1444393pubmed:dateRevised2010-9-7lld:pubmed
pubmed-article:1444393pubmed:meshHeadingpubmed-meshheading:1444393-...lld:pubmed
pubmed-article:1444393pubmed:meshHeadingpubmed-meshheading:1444393-...lld:pubmed
pubmed-article:1444393pubmed:meshHeadingpubmed-meshheading:1444393-...lld:pubmed
pubmed-article:1444393pubmed:meshHeadingpubmed-meshheading:1444393-...lld:pubmed
pubmed-article:1444393pubmed:meshHeadingpubmed-meshheading:1444393-...lld:pubmed
pubmed-article:1444393pubmed:meshHeadingpubmed-meshheading:1444393-...lld:pubmed
pubmed-article:1444393pubmed:meshHeadingpubmed-meshheading:1444393-...lld:pubmed
pubmed-article:1444393pubmed:meshHeadingpubmed-meshheading:1444393-...lld:pubmed
pubmed-article:1444393pubmed:meshHeadingpubmed-meshheading:1444393-...lld:pubmed
pubmed-article:1444393pubmed:meshHeadingpubmed-meshheading:1444393-...lld:pubmed
pubmed-article:1444393pubmed:year1992lld:pubmed
pubmed-article:1444393pubmed:articleTitle16S rRNA-based probes and polymerase chain reaction method to detect Listeria monocytogenes cells added to foods.lld:pubmed
pubmed-article:1444393pubmed:affiliationDepartment of Food Science, University of Arkansas, Fayetteville 72703.lld:pubmed
pubmed-article:1444393pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:1444393pubmed:publicationTypeResearch Support, U.S. Gov't, Non-P.H.S.lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1444393lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1444393lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1444393lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1444393lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1444393lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1444393lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1444393lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1444393lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1444393lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1444393lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1444393lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1444393lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1444393lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1444393lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1444393lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1444393lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1444393lld:pubmed