| pubmed-article:1329020 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1329020 | lifeskim:mentions | umls-concept:C1518275 | lld:lifeskim |
| pubmed-article:1329020 | lifeskim:mentions | umls-concept:C0032005 | lld:lifeskim |
| pubmed-article:1329020 | lifeskim:mentions | umls-concept:C0027793 | lld:lifeskim |
| pubmed-article:1329020 | lifeskim:mentions | umls-concept:C1521827 | lld:lifeskim |
| pubmed-article:1329020 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:1329020 | lifeskim:mentions | umls-concept:C1519355 | lld:lifeskim |
| pubmed-article:1329020 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:1329020 | pubmed:dateCreated | 1992-10-29 | lld:pubmed |
| pubmed-article:1329020 | pubmed:abstractText | Patch-clamp recording techniques were applied to thin slices of the rat pituitary gland in order to study synaptic transmission between hypothalamic nerve terminals and neuroendocrine cells of the intermediate lobe. Inhibitory postsynaptic currents (IPSCs) could be evoked by electrical stimulation of afferent neuronal fibres in the surrounding tissue of the slice. The IPSCs could be evoked in an all-or-nothing mode depending on the stimulus intensity, suggesting that single afferent fibres were stimulated. They had a chloride-dependent reversal potential and were blocked by bicuculline (Kd = 0.1 microM), indicating that they were mediated by gamma-aminobutyric acid A (GABAA) receptors. In symmetrical chloride solutions the current/voltage relation of the IPSC peak amplitudes was linear. The IPSCs were characterized by a fast (1-2 ms) rise time and a biexponential decay, with time constants of 21 +/- 4 ms and 58 +/- 14 ms at a holding potential of -60 mV (n = 6 cells). Both decay time constants increased with depolarization in an exponential manner. Spontaneously occurring IPSCs had a time course that was similar to that of evoked IPSCs. These miniature IPSCs, recorded in 1 microM tetrodotoxin, displayed an amplitude distribution that was well fitted by single Gaussian functions, with a mean value of its maxima of 18.1 +/- 2.3 pA (n = 4 cells). Amplitude histograms of evoked IPSCs were characterized by multiple peaks with a modal amplitude of about 18 pA (n = 6 cells). These findings indicate the quantal nature of GABAergic synaptic transmission in this system, with a quantal conductance step of about 280 pS. Single-channel currents underlying the IPSCs were studied by bath application of GABA to outside-out patches excised from intermediate lobe cells. Such GABA-induced currents revealed two conductance levels of 14 pS and 26 pS. In conclusion, GABAergic synaptic transmission in neuroendocrine cells of the pituitary has properties that are quite similar to those observed in neurones of the central nervous system. | lld:pubmed |
| pubmed-article:1329020 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1329020 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1329020 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1329020 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1329020 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1329020 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:1329020 | pubmed:issn | 0031-6768 | lld:pubmed |
| pubmed-article:1329020 | pubmed:author | pubmed-author:KonnerthAA | lld:pubmed |
| pubmed-article:1329020 | pubmed:author | pubmed-author:Schneggenburg... | lld:pubmed |
| pubmed-article:1329020 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1329020 | pubmed:volume | 421 | lld:pubmed |
| pubmed-article:1329020 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1329020 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1329020 | pubmed:pagination | 364-73 | lld:pubmed |
| pubmed-article:1329020 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:1329020 | pubmed:meshHeading | pubmed-meshheading:1329020-... | lld:pubmed |
| pubmed-article:1329020 | pubmed:meshHeading | pubmed-meshheading:1329020-... | lld:pubmed |
| pubmed-article:1329020 | pubmed:meshHeading | pubmed-meshheading:1329020-... | lld:pubmed |
| pubmed-article:1329020 | pubmed:meshHeading | pubmed-meshheading:1329020-... | lld:pubmed |
| pubmed-article:1329020 | pubmed:meshHeading | pubmed-meshheading:1329020-... | lld:pubmed |
| pubmed-article:1329020 | pubmed:meshHeading | pubmed-meshheading:1329020-... | lld:pubmed |
| pubmed-article:1329020 | pubmed:meshHeading | pubmed-meshheading:1329020-... | lld:pubmed |
| pubmed-article:1329020 | pubmed:meshHeading | pubmed-meshheading:1329020-... | lld:pubmed |
| pubmed-article:1329020 | pubmed:meshHeading | pubmed-meshheading:1329020-... | lld:pubmed |
| pubmed-article:1329020 | pubmed:meshHeading | pubmed-meshheading:1329020-... | lld:pubmed |
| pubmed-article:1329020 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1329020 | pubmed:articleTitle | GABA-mediated synaptic transmission in neuroendocrine cells: a patch-clamp study in a pituitary slice preparation. | lld:pubmed |
| pubmed-article:1329020 | pubmed:affiliation | Max-Planck-Institut für biophysikalische Chemie, Göttingen, Federal Republic of Germany. | lld:pubmed |
| pubmed-article:1329020 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1329020 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1329020 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1329020 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1329020 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1329020 | lld:pubmed |
