| pubmed-article:1324919 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1324919 | lifeskim:mentions | umls-concept:C0162508 | lld:lifeskim |
| pubmed-article:1324919 | lifeskim:mentions | umls-concept:C0033640 | lld:lifeskim |
| pubmed-article:1324919 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:1324919 | lifeskim:mentions | umls-concept:C1519253 | lld:lifeskim |
| pubmed-article:1324919 | lifeskim:mentions | umls-concept:C1546857 | lld:lifeskim |
| pubmed-article:1324919 | pubmed:issue | 24 | lld:pubmed |
| pubmed-article:1324919 | pubmed:dateCreated | 1992-9-25 | lld:pubmed |
| pubmed-article:1324919 | pubmed:abstractText | The addition of phorbol esters to U937 leukemic cells stimulates the phosphorylation of c-Jun on serines 63 and 73. To isolate the protein kinase which stimulates this phosphorylation, we have used heparin-Sepharose chromatography followed by affinity chromatography over glutathione-Sepharose beads bound with a fusion protein of glutathione S-transferase and amino acids 5-89 of c-Jun (GST-c-Jun). Using this procedure we purify a 67-kDa protein which is capable of phosphorylating GST-c-Jun as well as the complete c-Jun protein. By making mutations in serines 63 and 73 and then creating a fusion protein with GST (GST-c-Jun mut), we demonstrate that this protein kinase specifically phosphorylates these sites in the c-Jun amino terminus. Treatment of purified c-Jun amino-terminal protein kinase (cJAT-PK) with phosphatase 2A inhibits its ability to phosphorylate GST-c-Jun. This inactivated enzyme can be reactivated by phosphorylation with protein kinase C (PKC), although PKC is not capable of phosphorylating the GST-c-Jun substrate. Because v-Jun cannot be phosphorylated in vivo, we compared the ability of cJAT-PK to bind to GST-v-Jun or GST-c-Jun mut. The cJAT-PK bound 50-fold better to GST-c-Jun mut than GST-v-Jun suggesting that the delta domain which is missing in v-Jun plays a role in binding the cJAT-PK. These results suggest that there is a protein kinase cascade mediated by protein phosphatases and PKC which regulates c-Jun phosphorylation. | lld:pubmed |
| pubmed-article:1324919 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1324919 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1324919 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1324919 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:1324919 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1324919 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:1324919 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:1324919 | pubmed:author | pubmed-author:WagnerFF | lld:pubmed |
| pubmed-article:1324919 | pubmed:author | pubmed-author:KraftA SAS | lld:pubmed |
| pubmed-article:1324919 | pubmed:author | pubmed-author:AdlerVV | lld:pubmed |
| pubmed-article:1324919 | pubmed:author | pubmed-author:PolotskayaAA | lld:pubmed |
| pubmed-article:1324919 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1324919 | pubmed:day | 25 | lld:pubmed |
| pubmed-article:1324919 | pubmed:volume | 267 | lld:pubmed |
| pubmed-article:1324919 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1324919 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1324919 | pubmed:pagination | 17001-5 | lld:pubmed |
| pubmed-article:1324919 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:1324919 | pubmed:meshHeading | pubmed-meshheading:1324919-... | lld:pubmed |
| pubmed-article:1324919 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1324919 | pubmed:articleTitle | Affinity-purified c-Jun amino-terminal protein kinase requires serine/threonine phosphorylation for activity. | lld:pubmed |
| pubmed-article:1324919 | pubmed:affiliation | Division of Hematology/Oncology, University of Alabama, Birmingham 35294. | lld:pubmed |
| pubmed-article:1324919 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1324919 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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