Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:1310990rdf:typepubmed:Citationlld:pubmed
pubmed-article:1310990lifeskim:mentionsumls-concept:C0014834lld:lifeskim
pubmed-article:1310990lifeskim:mentionsumls-concept:C0059946lld:lifeskim
pubmed-article:1310990lifeskim:mentionsumls-concept:C1148735lld:lifeskim
pubmed-article:1310990pubmed:issue6lld:pubmed
pubmed-article:1310990pubmed:dateCreated1992-3-24lld:pubmed
pubmed-article:1310990pubmed:abstractTextA fluorescence assay was used to measure the processivity of Escherichia coli recBCD enzyme helicase activity. Under standard conditions, recBCD enzyme unwinds an average of 30 +/- 3.2 kilobase pairs (kb)/DNA end before dissociating. The average processivity (P obs) of DNA unwinding under these conditions is 0.99997, indicating that the probability of unwinding another base pair is 30,000-fold greater than the probability of dissociating from the double-stranded DNA. The average number of base pairs unwound per binding event (N) is sensitive to both mono- and divalent salt concentration and ranges from 36 kb at 80 mM NaCl to 15 kb at 280 mM NaCl. The processivity of unwinding increases in a hyperbolic manner with increasing ATP concentration, yielding a KN value for ATP of 41 +/- 9 microM and a limiting value of 32 +/- 1.8 kb/end for the number of base pairs unwound. The importance of the processivity of recBCD enzyme helicase activity to the recBCD enzyme-dependent stimulation of recombination at Chi sites observed in vivo is discussed.lld:pubmed
pubmed-article:1310990pubmed:granthttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1310990pubmed:granthttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1310990pubmed:languageenglld:pubmed
pubmed-article:1310990pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1310990pubmed:citationSubsetIMlld:pubmed
pubmed-article:1310990pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1310990pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1310990pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1310990pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1310990pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1310990pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1310990pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1310990pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1310990pubmed:statusMEDLINElld:pubmed
pubmed-article:1310990pubmed:monthFeblld:pubmed
pubmed-article:1310990pubmed:issn0021-9258lld:pubmed
pubmed-article:1310990pubmed:authorpubmed-author:Kowalczykowsk...lld:pubmed
pubmed-article:1310990pubmed:authorpubmed-author:RomanL JLJlld:pubmed
pubmed-article:1310990pubmed:authorpubmed-author:EgglestonA...lld:pubmed
pubmed-article:1310990pubmed:issnTypePrintlld:pubmed
pubmed-article:1310990pubmed:day25lld:pubmed
pubmed-article:1310990pubmed:volume267lld:pubmed
pubmed-article:1310990pubmed:ownerNLMlld:pubmed
pubmed-article:1310990pubmed:authorsCompleteYlld:pubmed
pubmed-article:1310990pubmed:pagination4207-14lld:pubmed
pubmed-article:1310990pubmed:dateRevised2007-11-14lld:pubmed
pubmed-article:1310990pubmed:meshHeadingpubmed-meshheading:1310990-...lld:pubmed
pubmed-article:1310990pubmed:meshHeadingpubmed-meshheading:1310990-...lld:pubmed
pubmed-article:1310990pubmed:meshHeadingpubmed-meshheading:1310990-...lld:pubmed
pubmed-article:1310990pubmed:meshHeadingpubmed-meshheading:1310990-...lld:pubmed
pubmed-article:1310990pubmed:meshHeadingpubmed-meshheading:1310990-...lld:pubmed
pubmed-article:1310990pubmed:meshHeadingpubmed-meshheading:1310990-...lld:pubmed
pubmed-article:1310990pubmed:meshHeadingpubmed-meshheading:1310990-...lld:pubmed
pubmed-article:1310990pubmed:meshHeadingpubmed-meshheading:1310990-...lld:pubmed
pubmed-article:1310990pubmed:meshHeadingpubmed-meshheading:1310990-...lld:pubmed
pubmed-article:1310990pubmed:meshHeadingpubmed-meshheading:1310990-...lld:pubmed
pubmed-article:1310990pubmed:meshHeadingpubmed-meshheading:1310990-...lld:pubmed
pubmed-article:1310990pubmed:year1992lld:pubmed
pubmed-article:1310990pubmed:articleTitleProcessivity of the DNA helicase activity of Escherichia coli recBCD enzyme.lld:pubmed
pubmed-article:1310990pubmed:affiliationDepartment of Molecular Biology, Northwestern University Medical School, Chicago, Illinois 60611.lld:pubmed
pubmed-article:1310990pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:1310990pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1310990lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1310990lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1310990lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1310990lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1310990lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1310990lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1310990lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1310990lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1310990lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1310990lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1310990lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1310990lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1310990lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1310990lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1310990lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1310990lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1310990lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1310990lld:pubmed