| pubmed-article:12969762 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12969762 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:12969762 | lifeskim:mentions | umls-concept:C0031327 | lld:lifeskim |
| pubmed-article:12969762 | lifeskim:mentions | umls-concept:C0019564 | lld:lifeskim |
| pubmed-article:12969762 | lifeskim:mentions | umls-concept:C0009079 | lld:lifeskim |
| pubmed-article:12969762 | lifeskim:mentions | umls-concept:C0870883 | lld:lifeskim |
| pubmed-article:12969762 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:12969762 | pubmed:dateCreated | 2003-9-12 | lld:pubmed |
| pubmed-article:12969762 | pubmed:abstractText | Up to now, it is not yet clear whether and how clozapine and its metabolites are metabolized in neuronal cells. The interconversion of clozapine and its metabolites, clozapine-N-oxide and norclozapine, was studied in the hippocampal neuronal in vitro system of HT22 cells. Clinically relevant concentrations of clozapine (200+400 ng/ml) and its metabolites (100+200 ng/ml) were used for the examination of the metabolizing effects after short- (4 h) and long- (24 h) term incubation. Two-way analysis of variance revealed a significant decrease of clozapine (P<0.01) and norclozapine (P<0.01) levels in the supernatants of HT22 cells after the treatment procedures. Student-Newman-Keuls tests showed a significant decrease of clozapine 400 after 24 h of incubation (P=0.01) as well as of all concentrations of norclozapine. No significant treatment effects were found for the clozapine-N-oxide degradation. Using semi-quantification by reverse transcriptase-polymerase chain reaction methods, we could show a significant increase of cytochrome P450 (CYP) 1A2 mRNA levels (P<0.05) after clozapine treatment with 200 ng/ml. The results of the present study strongly suggest that clozapine and norclozapine are metabolized in hippocampal neuronal HT22 cells by CYP1A2, whereas the levels of clozapine-N-oxide were not affected. Moreover, CYP1A2 mRNA levels were significantly changed by incubation with clozapine 200. | lld:pubmed |
| pubmed-article:12969762 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12969762 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12969762 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12969762 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12969762 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12969762 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12969762 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12969762 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12969762 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12969762 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:12969762 | pubmed:issn | 0014-2999 | lld:pubmed |
| pubmed-article:12969762 | pubmed:author | pubmed-author:VedderHelmutH | lld:pubmed |
| pubmed-article:12969762 | pubmed:author | pubmed-author:Schüler-Sprin... | lld:pubmed |
| pubmed-article:12969762 | pubmed:author | pubmed-author:RemschmidtHel... | lld:pubmed |
| pubmed-article:12969762 | pubmed:author | pubmed-author:KriegJürgen-C... | lld:pubmed |
| pubmed-article:12969762 | pubmed:author | pubmed-author:HeiserPhilipP | lld:pubmed |
| pubmed-article:12969762 | pubmed:author | pubmed-author:HausmannChris... | lld:pubmed |
| pubmed-article:12969762 | pubmed:author | pubmed-author:SchulteEvaE | lld:pubmed |
| pubmed-article:12969762 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12969762 | pubmed:day | 29 | lld:pubmed |
| pubmed-article:12969762 | pubmed:volume | 476 | lld:pubmed |
| pubmed-article:12969762 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12969762 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12969762 | pubmed:pagination | 167-72 | lld:pubmed |
| pubmed-article:12969762 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:12969762 | pubmed:meshHeading | pubmed-meshheading:12969762... | lld:pubmed |
| pubmed-article:12969762 | pubmed:meshHeading | pubmed-meshheading:12969762... | lld:pubmed |
| pubmed-article:12969762 | pubmed:meshHeading | pubmed-meshheading:12969762... | lld:pubmed |
| pubmed-article:12969762 | pubmed:meshHeading | pubmed-meshheading:12969762... | lld:pubmed |
| pubmed-article:12969762 | pubmed:meshHeading | pubmed-meshheading:12969762... | lld:pubmed |
| pubmed-article:12969762 | pubmed:meshHeading | pubmed-meshheading:12969762... | lld:pubmed |
| pubmed-article:12969762 | pubmed:meshHeading | pubmed-meshheading:12969762... | lld:pubmed |
| pubmed-article:12969762 | pubmed:meshHeading | pubmed-meshheading:12969762... | lld:pubmed |
| pubmed-article:12969762 | pubmed:meshHeading | pubmed-meshheading:12969762... | lld:pubmed |
| pubmed-article:12969762 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12969762 | pubmed:articleTitle | Pharmacokinetics of clozapine and its metabolites in hippocampal HT22 cells. | lld:pubmed |
| pubmed-article:12969762 | pubmed:affiliation | Department of Child and Adolescent Psychiatry and Psychotherapy, Philipps-University of Marburg, Hans-Sachs-Str 6, 35037 Marburg, Germany. heiser@mailer.uni-marburg.de | lld:pubmed |
| pubmed-article:12969762 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12969762 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12969762 | lld:pubmed |
