| pubmed-article:12960973 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12960973 | lifeskim:mentions | umls-concept:C0335038 | lld:lifeskim |
| pubmed-article:12960973 | lifeskim:mentions | umls-concept:C2700384 | lld:lifeskim |
| pubmed-article:12960973 | lifeskim:mentions | umls-concept:C0026882 | lld:lifeskim |
| pubmed-article:12960973 | lifeskim:mentions | umls-concept:C0028953 | lld:lifeskim |
| pubmed-article:12960973 | lifeskim:mentions | umls-concept:C0017259 | lld:lifeskim |
| pubmed-article:12960973 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:12960973 | lifeskim:mentions | umls-concept:C1515655 | lld:lifeskim |
| pubmed-article:12960973 | pubmed:issue | 21 | lld:pubmed |
| pubmed-article:12960973 | pubmed:dateCreated | 2003-9-8 | lld:pubmed |
| pubmed-article:12960973 | pubmed:abstractText | Branched oligonucleotides (b-oligonucleotides) based on a novel branching monomer were used for site-specific sequence alteration in vivo. With a stable integrated mutated enhanced green fluorescent protein (EGFP) template in Chinese hamster ovary cells, up to 0.1% EGFP-positive cells were counted after transfection with b-oligonucleotides. The presence of EGFP protein in converted cells was demonstrated by anti-EGFP immunocytochemistry. Genomic sequencing of converted cells showed in 40% of the analysed clones the corrected wild-type codon, while 9.3% of the sequences showed a corrected wild-type sequence and an additional collateral mutation. Despite the stable corrected genomic locus, converted cells entered selective apoptosis after 3-6 days. The cell line Irs-1 that is deficient in the homologous recombination pathway showed a reduced frequency of b-oligonucleotide-induced site-specific sequence conversion. The reduced conversion rates in the mutant cell line could be partly rescued by complementation with XRCC2 cDNA. | lld:pubmed |
| pubmed-article:12960973 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12960973 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12960973 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12960973 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12960973 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12960973 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12960973 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:12960973 | pubmed:issn | 0969-7128 | lld:pubmed |
| pubmed-article:12960973 | pubmed:author | pubmed-author:McKeenCC | lld:pubmed |
| pubmed-article:12960973 | pubmed:author | pubmed-author:KrauseEE | lld:pubmed |
| pubmed-article:12960973 | pubmed:author | pubmed-author:OlsenP APA | lld:pubmed |
| pubmed-article:12960973 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12960973 | pubmed:volume | 10 | lld:pubmed |
| pubmed-article:12960973 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12960973 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12960973 | pubmed:pagination | 1830-40 | lld:pubmed |
| pubmed-article:12960973 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:12960973 | pubmed:meshHeading | pubmed-meshheading:12960973... | lld:pubmed |
| pubmed-article:12960973 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12960973 | pubmed:articleTitle | Branched oligonucleotides induce in vivo gene conversion of a mutated EGFP reporter. | lld:pubmed |
| pubmed-article:12960973 | pubmed:affiliation | Section for Genetic Therapy, Institute of Microbiology, The National Hospital, Oslo, Norway. | lld:pubmed |
| pubmed-article:12960973 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12960973 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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