| pubmed-article:12945897 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12945897 | lifeskim:mentions | umls-concept:C0524637 | lld:lifeskim |
| pubmed-article:12945897 | lifeskim:mentions | umls-concept:C0028630 | lld:lifeskim |
| pubmed-article:12945897 | lifeskim:mentions | umls-concept:C0012892 | lld:lifeskim |
| pubmed-article:12945897 | lifeskim:mentions | umls-concept:C0243126 | lld:lifeskim |
| pubmed-article:12945897 | lifeskim:mentions | umls-concept:C2003941 | lld:lifeskim |
| pubmed-article:12945897 | lifeskim:mentions | umls-concept:C0871161 | lld:lifeskim |
| pubmed-article:12945897 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:12945897 | pubmed:dateCreated | 2003-8-29 | lld:pubmed |
| pubmed-article:12945897 | pubmed:abstractText | The highly mutagenic nucleoside dP (6-(2-deoxy-beta-D-erythro-pentofuranosyl)-3,4-dihydro-6H,8H-pyrimido[4,5-c][1,2]oxazin-2-one) is a bicyclic analogue of N4-methoxy-2'-deoxycytidine. It exists as a mixture of its imino and amino tautomers in solution with a ratio of about 10:1 based on its tautomeric constant. The bicyclic nature of the heterocycle P restrains the amino substituent in an anti conformation and permits effective Watson-Crick base-pairing using either tautomer. The specificity of incorporation of dP by the 3'-5'-exonuclease-free Klenow fragment of DNA polymerase I (exo-free Klenow) has been studied using the 5'-(1-thio)triphosphate dPTP alphaS in combination with phosphorothioate-specific sequencing of the DNA products. The method provides a convenient qualitative assay for studying nucleotide incorporation and reveals for the first time a potential role for the minor tautomeric forms of the natural DNA bases in base misinsertion (substitution mutagenesis) during replication. | lld:pubmed |
| pubmed-article:12945897 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12945897 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12945897 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12945897 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12945897 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12945897 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12945897 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12945897 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12945897 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12945897 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12945897 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12945897 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:12945897 | pubmed:issn | 1477-0520 | lld:pubmed |
| pubmed-article:12945897 | pubmed:author | pubmed-author:HornbyDavid... | lld:pubmed |
| pubmed-article:12945897 | pubmed:author | pubmed-author:WilliamsDavid... | lld:pubmed |
| pubmed-article:12945897 | pubmed:author | pubmed-author:HamiltonAlan... | lld:pubmed |
| pubmed-article:12945897 | pubmed:author | pubmed-author:HarrisVictori... | lld:pubmed |
| pubmed-article:12945897 | pubmed:author | pubmed-author:SmithClifford... | lld:pubmed |
| pubmed-article:12945897 | pubmed:author | pubmed-author:CumminsW... | lld:pubmed |
| pubmed-article:12945897 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12945897 | pubmed:day | 21 | lld:pubmed |
| pubmed-article:12945897 | pubmed:volume | 1 | lld:pubmed |
| pubmed-article:12945897 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12945897 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12945897 | pubmed:pagination | 2070-4 | lld:pubmed |
| pubmed-article:12945897 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:12945897 | pubmed:meshHeading | pubmed-meshheading:12945897... | lld:pubmed |
| pubmed-article:12945897 | pubmed:meshHeading | pubmed-meshheading:12945897... | lld:pubmed |
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| pubmed-article:12945897 | pubmed:meshHeading | pubmed-meshheading:12945897... | lld:pubmed |
| pubmed-article:12945897 | pubmed:meshHeading | pubmed-meshheading:12945897... | lld:pubmed |
| pubmed-article:12945897 | pubmed:meshHeading | pubmed-meshheading:12945897... | lld:pubmed |
| pubmed-article:12945897 | pubmed:meshHeading | pubmed-meshheading:12945897... | lld:pubmed |
| pubmed-article:12945897 | pubmed:meshHeading | pubmed-meshheading:12945897... | lld:pubmed |
| pubmed-article:12945897 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12945897 | pubmed:articleTitle | Recognition of base-pairing by DNA polymerases during nucleotide incorporation: the properties of the mutagenic nucleotide dPTP alphaS. | lld:pubmed |
| pubmed-article:12945897 | pubmed:affiliation | Centre for Chemical Biology, Department of Chemistry, Krebs Institute, University of Sheffield, Sheffield, UK S3 7HF. | lld:pubmed |
| pubmed-article:12945897 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12945897 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
