| pubmed-article:12920239 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12920239 | lifeskim:mentions | umls-concept:C1522424 | lld:lifeskim |
| pubmed-article:12920239 | lifeskim:mentions | umls-concept:C0026336 | lld:lifeskim |
| pubmed-article:12920239 | lifeskim:mentions | umls-concept:C0126732 | lld:lifeskim |
| pubmed-article:12920239 | lifeskim:mentions | umls-concept:C1522564 | lld:lifeskim |
| pubmed-article:12920239 | lifeskim:mentions | umls-concept:C0021308 | lld:lifeskim |
| pubmed-article:12920239 | lifeskim:mentions | umls-concept:C0035126 | lld:lifeskim |
| pubmed-article:12920239 | lifeskim:mentions | umls-concept:C1334877 | lld:lifeskim |
| pubmed-article:12920239 | lifeskim:mentions | umls-concept:C0456389 | lld:lifeskim |
| pubmed-article:12920239 | lifeskim:mentions | umls-concept:C1517499 | lld:lifeskim |
| pubmed-article:12920239 | lifeskim:mentions | umls-concept:C2349209 | lld:lifeskim |
| pubmed-article:12920239 | lifeskim:mentions | umls-concept:C1549649 | lld:lifeskim |
| pubmed-article:12920239 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:12920239 | pubmed:dateCreated | 2003-8-15 | lld:pubmed |
| pubmed-article:12920239 | pubmed:abstractText | Nuclear factor-kappaB (NF-kappaB) plays a central role in myocardial ischemia-reperfusion (MI/R) injury. The inhibitory protein IkappaBalpha prevents its activation. We investigated the effects of adeno-associated viral vector-mediated IkappaBalpha gene transfer in MI/R injury. Male C57BL/6 mice were randomized to receive a recombinant adeno-associated virus (rAAV) encoding the gene for the NF-kappaB inhibitory protein IkappaBalpha (rAAV- IkappaBalpha) or the beta-galactosidase gene (a control and inert gene; rAAV-LacZ), both at a dose of 10(11) copies. Four weeks later anesthetized animals were subjected to total occlusion (45 minutes) of the left main coronary artery followed by 5 hours of reperfusion. MI/R produced a wide infarct size (IF/area-at-risk = 56 +/- 8%; IF/left ventricle = 44 +/- 5%) and tissue neutrophil infiltration, studied by means of elastase activity (area-at-risk = 2.5 +/- 0.4 micro g/gm tissue; infarct area = 2.9 +/- 0.6 micro g/gm tissue). Furthermore MI/R caused peak message for intercellular adhesion molecule-1 (ICAM-1) in the area-at-risk at 3 hours of reperfusion (1.2 +/- 0.4 relative amount of cardiac ICAM-1 mRNA). NF-kappaB activation was evident at 0.5 hours of reperfusion and reached its maximum increase at 2 hours of reperfusion. rAAV-IkappaBalpha injection reduced infarct size (IF/area-at-risk = 19 +/- 3%; IF/left ventricle = 10 +/- 2%; p < 0.001), blocked NF-kappaB activation, diminished cardiac ICAM-1 expression (0.4 +/- 0.02 relative amount of cardiac ICAM-1 mRNA; p < 0.001), and blunted leukocyte accumulation (area-at-risk = 0.6 +/- 0.05 micro g/gm tissue; infarct area = 0.4 +/- 0.02 micro g/gm tissue; p < 0.001). Our data indicate that rAAV-IkappaBalpha may be useful for MI/R gene therapy. | lld:pubmed |
| pubmed-article:12920239 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12920239 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12920239 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12920239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12920239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12920239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12920239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12920239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12920239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12920239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12920239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12920239 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12920239 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:12920239 | pubmed:issn | 0023-6837 | lld:pubmed |
| pubmed-article:12920239 | pubmed:author | pubmed-author:AltavillaDome... | lld:pubmed |
| pubmed-article:12920239 | pubmed:author | pubmed-author:SquadritoGiov... | lld:pubmed |
| pubmed-article:12920239 | pubmed:author | pubmed-author:GaleanoMariar... | lld:pubmed |
| pubmed-article:12920239 | pubmed:author | pubmed-author:SquadritoFran... | lld:pubmed |
| pubmed-article:12920239 | pubmed:author | pubmed-author:GiaccaMauroM | lld:pubmed |
| pubmed-article:12920239 | pubmed:author | pubmed-author:MinutoliLette... | lld:pubmed |
| pubmed-article:12920239 | pubmed:author | pubmed-author:DeodatoBarbar... | lld:pubmed |
| pubmed-article:12920239 | pubmed:author | pubmed-author:SeminaraPaolo... | lld:pubmed |
| pubmed-article:12920239 | pubmed:author | pubmed-author:PassanitiMari... | lld:pubmed |
| pubmed-article:12920239 | pubmed:author | pubmed-author:MariniHerbert... | lld:pubmed |
| pubmed-article:12920239 | pubmed:author | pubmed-author:VenutiFrances... | lld:pubmed |
| pubmed-article:12920239 | pubmed:author | pubmed-author:MariniRolando... | lld:pubmed |
| pubmed-article:12920239 | pubmed:author | pubmed-author:AdamoElena... | lld:pubmed |
| pubmed-article:12920239 | pubmed:author | pubmed-author:BellomoMariaM | lld:pubmed |
| pubmed-article:12920239 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12920239 | pubmed:volume | 83 | lld:pubmed |
| pubmed-article:12920239 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12920239 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12920239 | pubmed:pagination | 1097-104 | lld:pubmed |
| pubmed-article:12920239 | pubmed:dateRevised | 2005-11-17 | lld:pubmed |
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| pubmed-article:12920239 | pubmed:meshHeading | pubmed-meshheading:12920239... | lld:pubmed |
| pubmed-article:12920239 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12920239 | pubmed:articleTitle | Gene transfer of IkappaBalpha limits infarct size in a mouse model of myocardial ischemia-reperfusion injury. | lld:pubmed |
| pubmed-article:12920239 | pubmed:affiliation | Department of Clinical and Experimental Medicine and Pharmacology, University of Messina, Messina, Italy. Francesco.Squadrito@unime.it | lld:pubmed |
| pubmed-article:12920239 | pubmed:publicationType | Journal Article | lld:pubmed |
| entrez-gene:18035 | entrezgene:pubmed | pubmed-article:12920239 | lld:entrezgene |
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