| pubmed-article:12912907 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12912907 | lifeskim:mentions | umls-concept:C0965644 | lld:lifeskim |
| pubmed-article:12912907 | lifeskim:mentions | umls-concept:C0542559 | lld:lifeskim |
| pubmed-article:12912907 | lifeskim:mentions | umls-concept:C0441889 | lld:lifeskim |
| pubmed-article:12912907 | lifeskim:mentions | umls-concept:C1333897 | lld:lifeskim |
| pubmed-article:12912907 | lifeskim:mentions | umls-concept:C1704838 | lld:lifeskim |
| pubmed-article:12912907 | lifeskim:mentions | umls-concept:C0205251 | lld:lifeskim |
| pubmed-article:12912907 | lifeskim:mentions | umls-concept:C1709379 | lld:lifeskim |
| pubmed-article:12912907 | lifeskim:mentions | umls-concept:C1554080 | lld:lifeskim |
| pubmed-article:12912907 | lifeskim:mentions | umls-concept:C1706198 | lld:lifeskim |
| pubmed-article:12912907 | pubmed:issue | 16 | lld:pubmed |
| pubmed-article:12912907 | pubmed:dateCreated | 2003-8-12 | lld:pubmed |
| pubmed-article:12912907 | pubmed:abstractText | Hypoxia-inducible factor (HIF), a transcriptional complex conserved from Caenorhabditis elegans to vertebrates, plays a pivotal role in cellular adaptation to low oxygen availability. In normoxia, the HIF-alpha subunits are targeted for destruction by prolyl hydroxylation, a specific modification that provides recognition for the E3 ubiquitin ligase complex containing the von Hippel-Lindau tumour suppressor protein (pVHL). Three HIF prolyl-hydroxylases (PHD1, 2 and 3) were identified recently in mammals and shown to hydroxylate HIF-alpha subunits. Here we show that specific 'silencing' of PHD2 with short interfering RNAs is sufficient to stabilize and activate HIF-1alpha in normoxia in all the human cells investigated. 'Silencing' of PHD1 and PHD3 has no effect on the stability of HIF-1alpha either in normoxia or upon re-oxygenation of cells briefly exposed to hypoxia. We therefore conclude that, in vivo, PHDs have distinct assigned functions, PHD2 being the critical oxygen sensor setting the low steady-state levels of HIF-1alpha in normoxia. Interestingly, PHD2 is upregulated by hypoxia, providing an HIF-1-dependent auto-regulatory mechanism driven by the oxygen tension. | lld:pubmed |
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| pubmed-article:12912907 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12912907 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12912907 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12912907 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12912907 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12912907 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12912907 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12912907 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12912907 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12912907 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:12912907 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12912907 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12912907 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:12912907 | pubmed:issn | 0261-4189 | lld:pubmed |
| pubmed-article:12912907 | pubmed:author | pubmed-author:PouysségurJac... | lld:pubmed |
| pubmed-article:12912907 | pubmed:author | pubmed-author:VolmatVéroniq... | lld:pubmed |
| pubmed-article:12912907 | pubmed:author | pubmed-author:BerraEdurneE | lld:pubmed |
| pubmed-article:12912907 | pubmed:author | pubmed-author:BenizriEmmanu... | lld:pubmed |
| pubmed-article:12912907 | pubmed:author | pubmed-author:GinouvèsAmand... | lld:pubmed |
| pubmed-article:12912907 | pubmed:author | pubmed-author:RouxDanièleD | lld:pubmed |
| pubmed-article:12912907 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12912907 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:12912907 | pubmed:volume | 22 | lld:pubmed |
| pubmed-article:12912907 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12912907 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12912907 | pubmed:pagination | 4082-90 | lld:pubmed |
| pubmed-article:12912907 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:12912907 | pubmed:meshHeading | pubmed-meshheading:12912907... | lld:pubmed |
| pubmed-article:12912907 | pubmed:meshHeading | pubmed-meshheading:12912907... | lld:pubmed |
| pubmed-article:12912907 | pubmed:meshHeading | pubmed-meshheading:12912907... | lld:pubmed |
| pubmed-article:12912907 | pubmed:meshHeading | pubmed-meshheading:12912907... | lld:pubmed |
| pubmed-article:12912907 | pubmed:meshHeading | pubmed-meshheading:12912907... | lld:pubmed |
