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pubmed-article:12745588pubmed:abstractTextIn vitro cultures of BM cells from newly diagnosed patients with AML displayed a defective BM stromal compartment, with a reduced number of fibroblast-colony-forming unit (CFU-F: 1 +/- 1.25 SD) and a decreased proliferative ability. The purposes of our study were: 1). to select BM mesenchymal stem cells (MSC) and BM-derived stromal cells (BMDSCs) from AML patients at diagnosis and from healthy subjects, using an immunomagnetic system and either anti-CD105 or anti-fibroblast MAbs; 2). to study the immunophenotypic and functional properties of freshly isolated and cultured mesenchymal cells; 3). to test the in vitro plasticity of the selected cells to differentiate towards an endothelial phenotype.lld:pubmed
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pubmed-article:12745588pubmed:pagination66-79lld:pubmed
pubmed-article:12745588pubmed:dateRevised2008-4-24lld:pubmed
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pubmed-article:12745588pubmed:articleTitleFunctional and immunophenotypic characteristics of isolated CD105(+) and fibroblast(+) stromal cells from AML: implications for their plasticity along endothelial lineage.lld:pubmed
pubmed-article:12745588pubmed:affiliationDepartment of Biomedical Sciences and Advanced Therapies University Hospital, Ferrara, Italy.lld:pubmed
pubmed-article:12745588pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:12745588pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed