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pubmed-article:12632395pubmed:abstractTextSpectrophotometric determination of laccase activity with ABTS acting as chromogen yields exceedingly low values whenever conducted in a water-organic mixed solvent. Nevertheless, there is firm evidence that laccase is able to oxidize substrates such as phenols and amines quantitatively in these mixed solvents. We show that the apparently small rate of ABTS oxidation by laccase in a mixed solvent, such as buffered water-dioxane 1:1, is not amenable to the denaturation of laccase but rather to the decreased stability of ABTS(.+). We propose HAA as a more reliable chromogen for the determination of laccase activity in mixed solvents.lld:pubmed
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pubmed-article:12632395pubmed:authorpubmed-author:GalliCarloClld:pubmed
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pubmed-article:12632395pubmed:authorpubmed-author:d'AcunzoFranc...lld:pubmed
pubmed-article:12632395pubmed:copyrightInfoCopyright 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 82: 395-398, 2003.lld:pubmed
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pubmed-article:12632395pubmed:pagination395-8lld:pubmed
pubmed-article:12632395pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:12632395pubmed:year2003lld:pubmed
pubmed-article:12632395pubmed:articleTitleDetermination of laccase activity in mixed solvents: comparison between two chromogens in a spectrophotometric assay.lld:pubmed
pubmed-article:12632395pubmed:affiliationDipartimento di Chimica and Centro CNR Meccanismi di Reazione, Università La Sapienza, 00185 Roma, Italy.lld:pubmed
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