| pubmed-article:12618486 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12618486 | lifeskim:mentions | umls-concept:C0021008 | lld:lifeskim |
| pubmed-article:12618486 | lifeskim:mentions | umls-concept:C0392747 | lld:lifeskim |
| pubmed-article:12618486 | lifeskim:mentions | umls-concept:C1148575 | lld:lifeskim |
| pubmed-article:12618486 | lifeskim:mentions | umls-concept:C0443172 | lld:lifeskim |
| pubmed-article:12618486 | lifeskim:mentions | umls-concept:C0332120 | lld:lifeskim |
| pubmed-article:12618486 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:12618486 | pubmed:dateCreated | 2003-3-5 | lld:pubmed |
| pubmed-article:12618486 | pubmed:abstractText | We have addressed the question of whether antigen binding induces a conformational change in the heavy chain constant (C(H)) domain of antibodies using staphylococcal protein A or streptococcal protein G as probes, since these proteins are known to bind to IgG domains such as C(H)1 and C(H)2-C(H)3 domains. Biosensor assays on interactions between these proteins and mouse IgG specific to (4-hydroxy-3-nitrophenyl)acetyl (NP) or their enzymatic fragments conducted in the presence or absence of the hapten, NP-epsilon-aminocaproic acid (NP-Cap), showed that the binding of IgG to these proteins was inhibited by the binding of NP-Cap. The results of isothermal titration calorimetry also revealed that the association constant for the interaction of protein A with IgG2b decreased by the addition of NP-Cap. These results suggested that antigen binding induced conformational changes in binding sites for protein G or protein A located at C(H)1 and C(H)2-C(H)3 domains, respectively. | lld:pubmed |
| pubmed-article:12618486 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12618486 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12618486 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12618486 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12618486 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12618486 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12618486 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12618486 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:12618486 | pubmed:issn | 0953-8178 | lld:pubmed |
| pubmed-article:12618486 | pubmed:author | pubmed-author:MoriiHisayuki... | lld:pubmed |
| pubmed-article:12618486 | pubmed:author | pubmed-author:OdaMasayukiM | lld:pubmed |
| pubmed-article:12618486 | pubmed:author | pubmed-author:AzumaTakachik... | lld:pubmed |
| pubmed-article:12618486 | pubmed:author | pubmed-author:KozonoHaruoH | lld:pubmed |
| pubmed-article:12618486 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12618486 | pubmed:volume | 15 | lld:pubmed |
| pubmed-article:12618486 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12618486 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12618486 | pubmed:pagination | 417-26 | lld:pubmed |
| pubmed-article:12618486 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:12618486 | pubmed:meshHeading | pubmed-meshheading:12618486... | lld:pubmed |
| pubmed-article:12618486 | pubmed:meshHeading | pubmed-meshheading:12618486... | lld:pubmed |
| pubmed-article:12618486 | pubmed:meshHeading | pubmed-meshheading:12618486... | lld:pubmed |
| pubmed-article:12618486 | pubmed:meshHeading | pubmed-meshheading:12618486... | lld:pubmed |
| pubmed-article:12618486 | pubmed:meshHeading | pubmed-meshheading:12618486... | lld:pubmed |
| pubmed-article:12618486 | pubmed:meshHeading | pubmed-meshheading:12618486... | lld:pubmed |
| pubmed-article:12618486 | pubmed:meshHeading | pubmed-meshheading:12618486... | lld:pubmed |
| pubmed-article:12618486 | pubmed:meshHeading | pubmed-meshheading:12618486... | lld:pubmed |
| pubmed-article:12618486 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12618486 | pubmed:articleTitle | Evidence of allosteric conformational changes in the antibody constant region upon antigen binding. | lld:pubmed |
| pubmed-article:12618486 | pubmed:affiliation | Research Institute for Biological Sciences, Tokyo University of Science, 2669 Noda, Chiba 278-0022, Japan. | lld:pubmed |
| pubmed-article:12618486 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12618486 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12618486 | lld:pubmed |
