| pubmed-article:12601078 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12601078 | lifeskim:mentions | umls-concept:C0036668 | lld:lifeskim |
| pubmed-article:12601078 | lifeskim:mentions | umls-concept:C0182400 | lld:lifeskim |
| pubmed-article:12601078 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:12601078 | pubmed:dateCreated | 2003-2-25 | lld:pubmed |
| pubmed-article:12601078 | pubmed:abstractText | The potential of photoaptamers as proteomic probes was investigated. Photoaptamers are defined as aptamers that bear photocross-linking functionality, in this report, 5-bromo-2'-deoxyuridine. A key question regarding the use of photoaptamer probes is the specificity of the cross-linking reaction. The specificity of three photoaptamers was explored by comparing their reactions with target proteins and non-target proteins. The range of target/non-target specificity varies from 100- to >10(6)-fold with most values >10(4)-fold. The contributions of the initial binding step and the photocross-linking step were evaluated for each reaction. Photocross-linking never degraded specificity and significantly increased aptamer specificity in some cases. The application of photoaptamer technology to proteomics was investigated in microarray format. Immobilized anti-human immunodeficiency virus-gp120 aptamer was able to detect subnanomolar concentrations of target protein in 5% human serum. The levels of sensitivity and specificity displayed by photoaptamers, combined with other advantageous properties of aptamers, should facilitate development of protein chip technology. | lld:pubmed |
| pubmed-article:12601078 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12601078 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12601078 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12601078 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12601078 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12601078 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12601078 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12601078 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12601078 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:12601078 | pubmed:issn | 1535-9476 | lld:pubmed |
| pubmed-article:12601078 | pubmed:author | pubmed-author:KochTad HTH | lld:pubmed |
| pubmed-article:12601078 | pubmed:author | pubmed-author:SmithDrewD | lld:pubmed |
| pubmed-article:12601078 | pubmed:author | pubmed-author:CollinsBrian... | lld:pubmed |
| pubmed-article:12601078 | pubmed:author | pubmed-author:HeilJamesJ | lld:pubmed |
| pubmed-article:12601078 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12601078 | pubmed:volume | 2 | lld:pubmed |
| pubmed-article:12601078 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12601078 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12601078 | pubmed:pagination | 11-8 | lld:pubmed |
| pubmed-article:12601078 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:12601078 | pubmed:meshHeading | pubmed-meshheading:12601078... | lld:pubmed |
| pubmed-article:12601078 | pubmed:meshHeading | pubmed-meshheading:12601078... | lld:pubmed |
| pubmed-article:12601078 | pubmed:meshHeading | pubmed-meshheading:12601078... | lld:pubmed |
| pubmed-article:12601078 | pubmed:meshHeading | pubmed-meshheading:12601078... | lld:pubmed |
| pubmed-article:12601078 | pubmed:meshHeading | pubmed-meshheading:12601078... | lld:pubmed |
| pubmed-article:12601078 | pubmed:meshHeading | pubmed-meshheading:12601078... | lld:pubmed |
| pubmed-article:12601078 | pubmed:meshHeading | pubmed-meshheading:12601078... | lld:pubmed |
| pubmed-article:12601078 | pubmed:meshHeading | pubmed-meshheading:12601078... | lld:pubmed |
| pubmed-article:12601078 | pubmed:meshHeading | pubmed-meshheading:12601078... | lld:pubmed |
| pubmed-article:12601078 | pubmed:meshHeading | pubmed-meshheading:12601078... | lld:pubmed |
| pubmed-article:12601078 | pubmed:meshHeading | pubmed-meshheading:12601078... | lld:pubmed |
| pubmed-article:12601078 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12601078 | pubmed:articleTitle | Sensitivity and specificity of photoaptamer probes. | lld:pubmed |
| pubmed-article:12601078 | pubmed:affiliation | SomaLogic, Inc, Boulder, Colorado 80301, USA. drew.smith@somalogic.com | lld:pubmed |
| pubmed-article:12601078 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12601078 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12601078 | lld:pubmed |
