| pubmed-article:12540526 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12540526 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:12540526 | lifeskim:mentions | umls-concept:C1882878 | lld:lifeskim |
| pubmed-article:12540526 | lifeskim:mentions | umls-concept:C0597357 | lld:lifeskim |
| pubmed-article:12540526 | lifeskim:mentions | umls-concept:C0178719 | lld:lifeskim |
| pubmed-article:12540526 | lifeskim:mentions | umls-concept:C0201925 | lld:lifeskim |
| pubmed-article:12540526 | lifeskim:mentions | umls-concept:C0006100 | lld:lifeskim |
| pubmed-article:12540526 | lifeskim:mentions | umls-concept:C0442805 | lld:lifeskim |
| pubmed-article:12540526 | lifeskim:mentions | umls-concept:C0449560 | lld:lifeskim |
| pubmed-article:12540526 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:12540526 | pubmed:dateCreated | 2003-1-23 | lld:pubmed |
| pubmed-article:12540526 | pubmed:abstractText | 1. RT-PCR and Western blots were used to detect bradykinin B(2) receptors in testis and isolated peritubular cells of pre-pubertal rats. RT-PCR demonstrated expression of a single transcript, whereas Western blots showed up to three specific bands that were in accordance with the described native, glycosylated and dimeric form of B(2) receptor proteins, respectively. 2. Fura-2-loaded peritubular cells responded with an instantaneous, linear and transient rise in [Ca(2+)](i) after adding bradykinin. Stimulation of cells with bradykinin concentrations between 1 micro M and 1 pM showed a dose dependent increase of [Ca(2+)](i). The calcium response to bradykinin was diminished after stimulation of peritubular cells in calcium-free buffer. After blocking the SERCA-pumps by thapsigargin and subsequent stimulation with bradykinin, no rise of [Ca(2+)](i) was appreciated. 3. Multiple stimulation of a single peritubular cell by local perfusion with a brief addition of BK (10 nM) resulted in a fast and immediate response. However, the second and third stimuli had slower rise rates and diminished [Ca(2+)](i) peaks, showing desensitization of the kinin receptor. 4. Addition of the bradykinin B(1) receptor agonist [des-Arg(9)]-bradykinin (100 nM) to Fura-2-loaded peritubular cells did not change the [Ca(2+)](i). However, the B(2) receptor antagonist HOE 140 (100 nM) strongly inhibited the bradykinin-induced calcium response. 5. We conclude that the bradykinin-induced increase in [Ca(2+)](i), in testicular peritubular cells is mediated by the stimulation of kinin receptors of the B(2) subtype. | lld:pubmed |
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| pubmed-article:12540526 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12540526 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12540526 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12540526 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:12540526 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12540526 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:12540526 | pubmed:issn | 0007-1188 | lld:pubmed |
| pubmed-article:12540526 | pubmed:author | pubmed-author:AumüllerGerha... | lld:pubmed |
| pubmed-article:12540526 | pubmed:author | pubmed-author:SchillWolf-Be... | lld:pubmed |
| pubmed-article:12540526 | pubmed:author | pubmed-author:WennemuthGunt... | lld:pubmed |
| pubmed-article:12540526 | pubmed:author | pubmed-author:MonseesThomas... | lld:pubmed |
| pubmed-article:12540526 | pubmed:author | pubmed-author:BlöcherSonjaS | lld:pubmed |
| pubmed-article:12540526 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12540526 | pubmed:volume | 138 | lld:pubmed |
| pubmed-article:12540526 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12540526 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12540526 | pubmed:pagination | 351-8 | lld:pubmed |
| pubmed-article:12540526 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:12540526 | pubmed:meshHeading | pubmed-meshheading:12540526... | lld:pubmed |
| pubmed-article:12540526 | pubmed:meshHeading | pubmed-meshheading:12540526... | lld:pubmed |
| pubmed-article:12540526 | pubmed:meshHeading | pubmed-meshheading:12540526... | lld:pubmed |
| pubmed-article:12540526 | pubmed:meshHeading | pubmed-meshheading:12540526... | lld:pubmed |
| pubmed-article:12540526 | pubmed:meshHeading | pubmed-meshheading:12540526... | lld:pubmed |
| pubmed-article:12540526 | pubmed:meshHeading | pubmed-meshheading:12540526... | lld:pubmed |
| pubmed-article:12540526 | pubmed:meshHeading | pubmed-meshheading:12540526... | lld:pubmed |
| pubmed-article:12540526 | pubmed:meshHeading | pubmed-meshheading:12540526... | lld:pubmed |
| pubmed-article:12540526 | pubmed:meshHeading | pubmed-meshheading:12540526... | lld:pubmed |
| pubmed-article:12540526 | pubmed:meshHeading | pubmed-meshheading:12540526... | lld:pubmed |
| pubmed-article:12540526 | pubmed:meshHeading | pubmed-meshheading:12540526... | lld:pubmed |
| pubmed-article:12540526 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12540526 | pubmed:articleTitle | Bradykinin increases intracellular calcium levels in rat testis peritubular cells via the B2 receptor subtype. | lld:pubmed |
| pubmed-article:12540526 | pubmed:affiliation | Department of Anatomy and Cell Biology, Philipps University, Robert-Koch-Str. 6, D-35037 Marburg, Germany. | lld:pubmed |
| pubmed-article:12540526 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12540526 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12540526 | lld:pubmed |
