Linked Life Data

12515911

Source:http://linkedlifedata.com/resource/pubmed/id/12515911

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pubmed-article:12515911pubmed:abstractTextTwo factors were found in the condition medium of neonatal pig liver fragments, which were capable of stimulating DNA synthesis in primary hepatocytes. They were named hepatocyte proliferation factor (HPF)-1 and HPF-2 and purified 1,025- and 2,580-fold, respectively. Both HPF-1 and HPF-2 seem to be anionic at pH 8.0 judged from the elution pattern of DEAE (DE52) column chromatography. HPF-1 was recovered as a non-adsorbed fraction in blue Sepharose and heparin Sepharose columns, and had a molecular weight of 26-31 kDa as estimated by gel filtration in high salt condition. Purified HPF-1 stimulated DNA synthesis of primary rat hepatocytes, but suppressed that of HepG2 cells. HPF-2 strongly bound to blue Sepharose and heparin Sepharose columns, and had a molecular weight of 71-90 kDa as estimated by SDS-PAGE under non-reduced condition. Purified HPF-2 stimulated DNA synthesis of primary rat hepatocytes dose dependently but did not suppress that of HepG2 cells. From further biological and chemical characteristics studied in this paper, HPF-1 and HPF-2 may be novel stimulating proteins for hepatocyte proliferation, although the possibility that they are already known growth factors can not be excluded without complete purification and its cloning.lld:pubmed
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pubmed-article:12515911pubmed:authorpubmed-author:TsudaMichioMlld:pubmed
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pubmed-article:12515911pubmed:dateRevised2008-11-21lld:pubmed
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pubmed-article:12515911pubmed:year2002lld:pubmed
pubmed-article:12515911pubmed:articleTitleHepatocyte proliferation factors from neonatal pig liver: purification and characterization.lld:pubmed
pubmed-article:12515911pubmed:affiliationDepartment of Molecular Life Science, Tokai University School of Medicine, Isehara, Kanagawa, 259-1193 Japan.lld:pubmed
pubmed-article:12515911pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:12515911pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed