| pubmed-article:12512699 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12512699 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:12512699 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:12512699 | lifeskim:mentions | umls-concept:C0021753 | lld:lifeskim |
| pubmed-article:12512699 | lifeskim:mentions | umls-concept:C0225336 | lld:lifeskim |
| pubmed-article:12512699 | lifeskim:mentions | umls-concept:C0041637 | lld:lifeskim |
| pubmed-article:12512699 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:12512699 | lifeskim:mentions | umls-concept:C0243769 | lld:lifeskim |
| pubmed-article:12512699 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:12512699 | pubmed:issue | 4 Pt 1 | lld:pubmed |
| pubmed-article:12512699 | pubmed:dateCreated | 2003-1-6 | lld:pubmed |
| pubmed-article:12512699 | pubmed:abstractText | Prostaglandin endoperoxide H2 (PGH2) is generated from arachidonic acid by either constitutive (COX-1) or inducible (COX-2) cyclooxygenases. In arterial wall PGH2 is converted by PGI2 synthase (PGI-S) to prostacyclin (PGI2), and in platelets by thromboxane synthase (TX-S) to thromboxane (TXA2). Other prostanoids as PGD2, PGF2alpha, or PGE2 were believed to arise non-enzymatically from PGH2. Only recently, human prostaglandin E synthase (PGE-S) has been identified and cloned as a membrane bound, microsomal, glutathione-dependent inducible enzyme. Here we demonstrated that interleukin 1beta (IL-1beta) is an inducer of COX-2 and PGE-S in human umbilical vein endothelial cells (HUVEC). Functional expression of PGE-S was measured at the level of specific mRNA by semi-quantitative RT-PCR, PGE-S protein was detected by Western blot in HUVEC, while PGE2 was measured by immunoassay in the supernatant. Actinomycin D, a classical transcription inhibitor, was used to prove that indeed IL-1beta induced the functional PGE-S enzyme. PGE2 generation in HUVEC was inhibited by indomethacin, acetaminophen and dexamethasone. In conclusion, we found that in cultured endothelial cells IL-1beta induced as evidenced by the appearance of its transcript and its functional enzyme. The induction of endothelial PGE-S and COX-2 appeared to be and their transcripts were induced as fast as one might expect from immediate early genes. It means that IL-1beta-triggered-PGE2 biosynthesis in endothelial cells is probably regulated by induction of both COX-2 and PGE-S. This is way we hypothesise the existence of at least two distinct pools of COX-2: the first selectively coupled to PGE-S and the second one that is coupled to PGI-S yielding the main endothelial product--PGI2. | lld:pubmed |
| pubmed-article:12512699 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12512699 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12512699 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12512699 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12512699 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12512699 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12512699 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12512699 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12512699 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12512699 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12512699 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12512699 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12512699 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12512699 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:12512699 | pubmed:issn | 0867-5910 | lld:pubmed |
| pubmed-article:12512699 | pubmed:author | pubmed-author:GryglewskiR... | lld:pubmed |
| pubmed-article:12512699 | pubmed:author | pubmed-author:UraczWW | lld:pubmed |
| pubmed-article:12512699 | pubmed:author | pubmed-author:OlszaneckiRR | lld:pubmed |
| pubmed-article:12512699 | pubmed:author | pubmed-author:UraczDD | lld:pubmed |
| pubmed-article:12512699 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12512699 | pubmed:volume | 53 | lld:pubmed |
| pubmed-article:12512699 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12512699 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12512699 | pubmed:pagination | 643-54 | lld:pubmed |
| pubmed-article:12512699 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:12512699 | pubmed:meshHeading | pubmed-meshheading:12512699... | lld:pubmed |
| pubmed-article:12512699 | pubmed:meshHeading | pubmed-meshheading:12512699... | lld:pubmed |
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| pubmed-article:12512699 | pubmed:meshHeading | pubmed-meshheading:12512699... | lld:pubmed |
| pubmed-article:12512699 | pubmed:meshHeading | pubmed-meshheading:12512699... | lld:pubmed |
| pubmed-article:12512699 | pubmed:meshHeading | pubmed-meshheading:12512699... | lld:pubmed |
| pubmed-article:12512699 | pubmed:meshHeading | pubmed-meshheading:12512699... | lld:pubmed |
| pubmed-article:12512699 | pubmed:meshHeading | pubmed-meshheading:12512699... | lld:pubmed |
| pubmed-article:12512699 | pubmed:meshHeading | pubmed-meshheading:12512699... | lld:pubmed |
| pubmed-article:12512699 | pubmed:meshHeading | pubmed-meshheading:12512699... | lld:pubmed |
| pubmed-article:12512699 | pubmed:meshHeading | pubmed-meshheading:12512699... | lld:pubmed |
| pubmed-article:12512699 | pubmed:meshHeading | pubmed-meshheading:12512699... | lld:pubmed |
| pubmed-article:12512699 | pubmed:meshHeading | pubmed-meshheading:12512699... | lld:pubmed |
| pubmed-article:12512699 | pubmed:year | 2002 | lld:pubmed |
| pubmed-article:12512699 | pubmed:articleTitle | Interleukin 1beta induces functional prostaglandin E synthase in cultured human umbilical vein endothelial cells. | lld:pubmed |
| pubmed-article:12512699 | pubmed:affiliation | Molecular Pharmacology Laboratory, Chair of Pharmacology, Medical College of Jagiellonian University, Grzegórzecka 16 31-531 Krakow, Poland. miuracz@kinga.cyf-kr.edu.pl | lld:pubmed |
| pubmed-article:12512699 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12512699 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:9536 | entrezgene:pubmed | pubmed-article:12512699 | lld:entrezgene |
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